智能水平旋转式细胞培养装置设计  

Design of intelligent horizontal rotating cell culture device

作　　者：杨丽斐 钱叶蓉 向俊西[2] 史爱华[1] 刘昕 魏沙沙 吕毅[1,2,4] 刘鹏 YANG Li-fei;QIAN Ye-rong;XIANG Jun-xi;SHI Ai-hua;LIU Xin;WEI Sha-sha;LYU Yi;LIU Peng(National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China;Department of Hepatobiliary Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China;Department of Graduate School,Xi'an Medical University,Xi'an 710021,China;Center for Regenerative and Reconstructive Medicine of Med-X Institute,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710048,China)

机构地区：[1]西安交通大学第一附属医院陕西省再生医学与外科工程研究中心,西安710061 [2]西安交通大学第一附属医院肝胆外科,西安710061 [3]西安医学院研究生院,西安710021 [4]西安交通大学第一附属医院Med-X研究院再生与重建医学研究所,西安710048

出　　处：《医疗卫生装备》2024年第9期41-45,共5页Chinese Medical Equipment Journal

基　　金：陕西省自然科学基金项目(2024JC-YBMS-653);陕西省自然科学基础研究计划项目(2022JQ-899&2021J M-268);陕西省创新能力支撑计划项目(2023KJXX-030);陕西省卫生计生委计划项目(2021E018)。

摘　　要：目的:研制一种模块化、操作简单、易消毒、成本低、稳定性高的智能水平旋转式细胞培养装置,以用于管状支架内表面的细胞三维动态培养。方法:该装置由旋转培养模块、驱动模块、控制模块和控制软件组成,每个模块的外壳均由3D打印制作。其中,旋转培养模块由管状静电纺丝支架、细胞培养仓、磁耦合转子、聚丙烯管道等组成;驱动模块由N20减速电机、磁耦合转子组成;控制模块由ESP-8266芯片及印刷电路板组成;控制软件通过Blinker物联网平台开发,采用C++语言编程。采用该装置培养人肝内胆管上皮细胞,验证该装置的应用效果。结果:光镜和扫描电子显微镜图像显示,在管状静电纺丝支架表面形成了均匀连续的细胞层。结论:该智能水平旋转式细胞培养装置可实现管状静电纺丝支架内表面细胞的均匀生长,为管状支架上的细胞培养提供了有效平台。Objective To develop an intelligent horizontal rotating cell culture device with high modularity,easy operation,easy disinfection,low cost and high stability.Methods The cell culture device consisted of a rotating culture module,a dirve module,a control module and control software,with the shells of all the modules being manufactured by 3D printing.The rotating culture module was composed of a tubular electrospun scaffold,a cell culture chamber,a magnetic coupling rotor and polypropylene pipeline;the drive module was made up of a N20 reduction motor and a magnetic coupling rotor;the control module included an ESP-8266 chip and a printed circuit board;the control software was developed with Blinker IoT platform and C++language.The device was used to culture human intrahepatic bile duct epithelial cells to verify its effects.Results Light microscopy and scanning electron microscopy images showed that a uniform and continuous cell layer was formed on the surface of the tubular electrospun scaffold.Conclusion The intelligent horizontal rotating cell culture device achieves uniform growth of cells on the inner surface of tubular electrospun scaffolds,and can be used as an effective platform for cell culture on tubular scaffolds.

关 键 词：旋转培养 细胞培养 细胞培养装置 管状静电纺丝支架 人工胆管 

分 类 号：Q813.11[生物学—生物工程]