甜瓜KASP标记开发及指纹图谱构建  

Development of melon KASP markers and construction of fingerprints

作　　者：肖玉珍 张瑞青 张跃星 代雪 张勇[1] 王中元[1] 于蓉[2] 侯尹婕 张显[1] 魏春华[1] XIAO Yuzhen;ZHANG Ruiqing;ZHANG Yuexing;DAI Xue;ZHANG Yong;WANG Zhongyuan;YU Rong;HOU Yinjie;ZHANG Xian;WEI Chunhua(College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China;Institute of Horticulture,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750000,Ningxia,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]宁夏农林科学院园艺研究所,银川750000

出　　处：《中国瓜菜》2024年第9期9-17,共9页China Cucurbits And Vegetables

基　　金：2023年西甜瓜良种联合攻关;宁夏农林科学院高质量发展和生态保护科技创新示范项目(NGSB-2021-7)。

摘　　要：为丰富甜瓜分子标记类型,并为后续甜瓜种质、品种及种子纯度鉴定提供简便、高效的检测方法,分别在甜瓜每条染色体上挑选6个核心SNP位点,共72个标记,通过KASP技术对11份甜瓜种质进行基因分型,确定了45对具有较好分型效果的标记,标记设计成功率为62.5%。从45对分型较好的标记中挑选25个基因型分簇集中的最优标记,对31份甜瓜种质进行基因分型,明确了各种质在25个SNP位点的多态性,通过聚类分析,分成了3组,并构建了31份甜瓜种质的指纹图谱,为甜瓜种质资源利用和种子纯度鉴定提供了参考。To enrich spectrum of molecular markers in melon and offer a straightforward and efficient protocol for the subsequent identification of melon germplasm,variety,and seed purity,this investigation identified six pivotal single-nucleotide polymorphism(SNP)loci distributed across the melon genome,encompassing a total of 72 markers.Utilizing the KASP(kompetitive allele-specific PCR)assay,these markers were employed to genotype 11 melon germplasm accessions,resulting in the identification of 45 marker pairs exhibiting superior typing performance.The success rate of marker design reached 62.5%,and from these 45 pairs,the most optimal markers for the 25 identified genetic clusters were selected,and the polymorphisms of 31 muskmelon germplasm were divided into 3 groups,and the fingerprints of 31 muskmelon germplasm were constructed to provide reference for the utilization of muskmelon germplasm resources and seed purity identification.

关 键 词：甜瓜 KASP SNP 指纹图谱 

分 类 号：S652[农业科学—果树学]