当阳鱼腥草花叶病病原分子鉴定  

Molecular identification of mosaic disease on Houttuynia cordata Thumb.in Dangyang

作　　者：周洁 吴方华 燕飞 齐传东 郭凤领[1] 吴金平[1] ZHOU Jie;WU Fanghua;YAN Fei;QI Chuandong;GUO Fengling;WU Jinping(Institute of Economic Crops,Hubei Academy of Agricultural Sciences/Key Laboratory of Vegetable Ecological Cultivation on Highland,Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement,Wuhan 430064,Hubei,China;Agricultural Service Center of Lianghe Town,Dangyang City,Yichang 444114,Hubei,China;Institute of Plant Virology,Ningbo University,Ningbo 315211,Zhejiang,China)

机构地区：[1]湖北省农业科学院经济作物研究所·农业农村部高山蔬菜生态栽培重点实验室·湖北省蔬菜种质创新与遗传改良重点实验室,武汉430064 [2]湖北省当阳市两河镇农业服务中心,湖北宜昌444114 [3]宁波大学植物病毒学研究所,浙江宁波315211

出　　处：《中国瓜菜》2024年第9期54-58,共5页China Cucurbits And Vegetables

基　　金：湖北省农业科技创新中心项目(2021-620-000-001-007);国家特色蔬菜产业技术体系(CARS-24-G-17)。

摘　　要：明确当阳鱼腥草花叶病致病菌种类,为当阳鱼腥草花叶病的田间防治提供依据。采集鱼腥草花叶叶片提取当阳鱼腥草典型病样的总RNA,利用sRNA深度测序,结合生物信息学分析初步确定病原菌,利用该病毒外壳蛋白(CP)特异性引物进行RT-PCR检测,所得序列进行BLAST比对和系统进化树构建,以进一步确定病原菌种类。利用sRNA深度测序和生物信息学初步确定鱼腥草花叶病致病菌为黄瓜花叶病毒(CMV),特异性引物扩增得到878 bp特异性条带,经BLAST比对发现其与已发表的CMV序列高度同源,相似性最高达97.84%。通过CMV病毒CP基因的核苷酸序列构建系统进化树可得鱼腥草花叶病病毒属于CMV亚组Ⅱ。引起鱼腥草花叶病的病原属于黄瓜花叶病毒Ⅱ亚组,研究结果为鱼腥草花叶病防治提供了理论基础。The identification of pathogenic bacteria species causing Houttuynia cordata mosaic disease in Dangyang will provide a theory basis for the field control of the disease.The mosaic leaves of Houttuynia cordata were collected to extract the total RNA of typical mosaic disease samples,and sRNA deep sequencing was conducted.Based on bioinformatics analysis,the pathogen was initially identified.Using the virus coat protein(CP)specific primers for RT-PCR detection,the obtained sequences were subjected to BLAST alignment and a phylogenetic tree construction to further identify the pathogen.Using sRNA deep sequencing and bioinformatics analysis,it was initially determined that the causative virus of Houttuynia cordata mosaic disease was cucumber mosaic virus(CMV).Specific primers amplified a specific band of 878 bp,which was found to be highly homologous to published CMV sequences through BLAST alignment,with a similarity of 97.84%.By constructing a phylogenetic tree based on the nucleotide sequence of CMV virus CP gene,it can be concluded that Houttuynia mosaic virus belongs to subgroup II of CMV.This result provides a theoretical basis for the prevention and treatment of Houttuynia cordata mosaic disease.

关 键 词：鱼腥草 黄瓜花叶病毒 sRNA深度测序 RT-PCR 

分 类 号：S649[农业科学—蔬菜学]