褪黑素浸种对辣椒种子萌发及幼苗抗旱性的影响  

Effects of melatonin soaking on pepper seed germination and seeding drought resistance

作　　者：马仲炼 周苓宇 陈悦 黄先敏 马永翠 史志龙 MAZhonglian;ZHOU Lingyu;CHEN Yue;HUANG Xianmin;MAYongcui;SHI Zhilong(College of Agronomy and Life Sciences,Zhaotong University,Zhaotong 657000,Yunnan,China;College of Agronomy and Biotechnology,Southwest University,Beibei 400716,Chongqing,China)

机构地区：[1]昭通学院农学与生命科学学院,云南昭通657000 [2]西南大学农学与生物科技学院,重庆北碚400716

出　　处：《中国瓜菜》2024年第9期128-135,共8页China Cucurbits And Vegetables

基　　金：云南省地方高校联合项目(202101BA070001-061);昭通市“兴昭人才支持计划”昭党人才〔2023〕3号。

摘　　要：为提高辣椒种子及幼苗的耐旱性,设置0(CK)、50μmol·L^(-1)( T1)、100μmol·L^(-1)( T2)、200μmol·L^(-1)( T3)褪黑素浸种和15%聚乙二醇(PEG 6000)模拟干旱,以贵农长线王辣椒为试验材料,研究褪黑素(melatonin)对辣椒种子萌发特性和干旱胁迫下幼苗抗旱性的影响。结果表明,褪黑素处理较CK处理能有效提高辣椒种子的发芽率9.06~9.96个百分点和存苗率4.69~5.78个百分点,促进辣椒幼苗生长;在干旱胁迫下,褪黑素处理较CK处理可以有效提高叶片相对含水量14.06~22.74个百分点、羟自由基清除能力3.95%~57.89%、总抗氧化能力23.73%~38.98%,降低细胞膜透性12.96%~22.24%、丙二醛含量9.12%~30.76%、过氧化氢含量5.90%~20.85%;但褪黑素处理较CK处理显著降低叶片可溶性糖含量63.05%~77.61%,降低脯氨酸含量4.43%~62.14%。灰色关联分析表明,褪黑素主要通过增加植株地下生物量、总抗氧化能力以及叶片相对含水量等来提高辣椒幼苗的抗旱性。通过抗旱系数分析表明,T1、T2和T3各处理平均抗旱系数为1.09、1.22和1.05,T2处理抗旱能力最强。抗旱性综合评价获得CK、T1、T2和T3处理的评价值为0.3380、0.6067、0.7700、0.5456。综上所述,褪黑素浸种能改善辣椒种子萌发生长特性,干旱胁迫下,褪黑素(100μmol·L^(-1))处理对辣椒抗旱能力提升最佳。To improve the drought tolerance of pepper seeds and seedlings,0(CK),50μmol·L^(-1)(T1),100μmol·L^(-1)(T2),200μmol·L^(-1)(T3)melatonin solution and 15%polyethylene glycol(PEG 6000)to simulate drought were set up,with Guinong Changxianwang as the experimental material to study the effect of melatonin on the germination characteristics of pepper seeds and drought resistance of seedlings under drought stress.The results showed that melatonin treatment could effectively increase the germination rate of pepper seeds by 9.06-9.96 percentage points and seedling survival rate by 4.69-5.78 percentage points,and promote the growth of pepper seedlings compared with CK treatment.Under drought stress,compared with CK treatment,melatonin treatment could effectively increase leaf water retention capacity by 14.06-22.74 percentage points,hydroxyl free radical scavenging capacity by 3.95%-57.89%,and total antioxidant capacity by 23.73%-38.98%,and reduce cell membrane permeability by 12.96%-22.24%,malondialdehyde content by 9.12%-30.76%,and hydrogen peroxide content by 5.90%-20.85%.However,melatonin treatment significantly reduced soluble sugar content by 63.05%-77.61%and proline content by 4.43%-62.14%compared with CK treatment.Grey correlation analysis showed that melatonin mainly improved drought resistance of pepper seedlings by increasing underground biomass,total antioxidant capacity and relative leaf water content.The analysis of drought resistance coefficient showed that the average drought resistance coefficient of T1,T2 and T3 were 1.09,1.22 and 1.05,respectively,with T2 having the strongest drought resistance.The comprehensive evaluation values of CK,T1,T2 and T3 treatments were 0.3380,0.6067,0.7700 and 0.5456.In summary,seed soaking with melatonin could improve the germination and growth characteristics of pepper seeds.Under drought stress,melatonin(100μmol·L^(-1))treatment had the best effect on drought resistance of pepper.

关 键 词：辣椒 褪黑素 浸种 萌发 抗旱性 

分 类 号：S641.3[农业科学—蔬菜学]