湖南省油茶良种根际微生物区系分析  

Analysis of Inter-root Microbiota of Improved Camellia oleifera Varieties in Hunan Province

作　　者：董文统 田诗义 叶佳伟 潘忠飞 袁德义[1] 周道顺[2] 刘慧敏[2] 邹锋[1] DONG Wen-tong;TIAN Shi-yi;YE Jia-wei;PAN Zhong-fei;YUAN De-yi;ZHOU Dao-shun;LIU Hui-min;ZOU Feng(College of Forest,Central South University of Forestry and Technology,Key Laboratory of Cultivation and Protection for Non-wood Forest Trees of Ministry Education,Key Lab of Non-wood Forest Products of State Forestry Administration,Changsha 410004,China;Research Institute of Non-timber Forestry,Chinese Academy of Forestry,Zhengzhou 450003,China)

机构地区：[1]中南林业科技大学林学院经济林培育与保护教育部重点实验室,经济林育种与栽培国家林草局重点实验室,湖南长沙410004 [2]中国林业科学研究院经济林研究所,河南郑州450003

出　　处：《土壤通报》2024年第4期1050-1059,共10页Chinese Journal of Soil Science

基　　金：国家重点研发计划项目(2022YFD2200400)和(2020YFD1000700)资助。

摘　　要：【目的】为了弄清湖南省油茶主栽良种根际微生物区系特征,筛选出关键差异微生物菌群,为油茶林土壤生物菌肥研发提供参考。【方法】利用高通量测序技术和OTU分析等方法,对湖南省油茶良种‘华硕’(HS)、‘华鑫’(HX)、‘湘林210’(XL210)以及普通油茶(CK)根际土壤微生物多样性及差异进行了分析,同时测定了油茶根际土壤理化性质及酶活性,运用冗余分析方法,探讨土壤因子与根际微生物之间的关系,并进行核心菌群的筛选。【结果】①油茶良种根际微生物数量的排序均为HS>HX>XL210>CK。铁、铜、总有机碳、铵态氮、β-葡萄糖苷酶是影响土壤细菌多样性的主要因子,全钾、电导率、植酸酶是影响土壤真菌多样性的主要因子。②油茶良种与对照存在显著性差异的微生物为Microscillaceae、那提普斯壳科Natipusillaceae、Leotiomycetes。【结论】差异微生物可作为人工合成菌群的核心候选成员,为油茶林土壤生物菌肥的研发奠定了基础。[Objective]The aims were to figure out the inter-root microbiota characteristics of Camellia oleifera varieties and screen out the key differential microflora in Hunan Province,which could provide an information for the development of soil biofertilizers in C.oleifera forests.[Methods]High throughput sequencing technology and OTU analysis were used to characterize the inter-root microbiota of C.oleifera varieties'Huashuo'(HS),'Huaxin'(HX),'Xianglin 210'(XL210),and CK.The physicochemical and enzyme activity properties of C.oleifera inter-root soils were also determined.The relationship between soil factors and inter-root microorganisms was investigated by redundancy analysis(RDA)and screened out the core flora in C.oleifera.[Results]①The order of the number of interroot microorganisms in C.oleifera varieties was HS>HX>XL210>CK.Fe,Cu,TOC,ammonium nitrogen,andβ-glucosidase were the main factors affecting the diversity of soil bacteria.Total potassium,electrical conductivity,and phytase were the main factors affecting the diversity of soil fungi.②Microorganisms with significant differences among different C.oleifera varieties were Microscillaceae,Natipusillaceae,unclassified_c_Leotiomycetes.[Conclusion]These differential microorganisms could be used as the core candidate members,and laid an foundation for development of the soil biofungus fertilizer in C.oleifera forests.

关 键 词：油茶 良种 根际微生物 关键微生物区系 

分 类 号：S154.37[农业科学—土壤学]