基于原位酶谱技术镉污染土壤青葙根际酶活性空间分布特征研究  

Spatial Distribution of Enzyme Activities in the Rhizosphere of Celosia argentea Linn.in Cd-contaminated Soil Using in-situ Soil Zymography

作　　者：刘泽蕙 刘杰[1,3] 舒毅 赖才星 蒋旭升 Asfandyar Shahab 俞果 LIU Ze-hui;LIU Jie;SHU Yi;LAI Cai-xing;JIANG Xu-sheng;Asfandyar Shahab;YU Guo(Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology,Guilin University of Technology,Guilin 541006,China;School of Environment,Tsinghua University,Beijing 100084,China;Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area,Guilin University of Technology,Guilin 541006,China)

机构地区：[1]桂林理工大学,广西环境污染控制理论与技术重点实验室,广西桂林541006 [2]清华大学环境学院,北京100084 [3]桂林理工大学,广西岩溶地区水污染控制与用水安全保障协同创新中心,广西桂林541006

出　　处：《土壤通报》2024年第4期1137-1142,共6页Chinese Journal of Soil Science

基　　金：国家自然科学基金(52200189);广西科技计划项目(2021GXNSFBA220055,GuikeAB21220049)。

摘　　要：【目的】为探明土壤镉污染对超富集植物青葙根际酶活性空间分布特征的影响。【方法】基于土壤酶谱技术,对镉污染土壤和非污染土壤中青葙根际β-葡萄糖苷酶、酸性磷酸酶分布情况进行原位高分辨分析。【结果】β-葡萄糖苷酶、酸性磷酸酶活性较高的热点区域都集中在青葙根系分布的区域,且在根尖处浓度较高。在污染土壤和非污染土壤中,青葙根部中心位置β-葡萄糖苷酶和酸性磷酸酶活性灰度值都显著高于距离根中心2 mm处酶活性灰度值。镉污染土壤青葙根部中心位置β-葡萄糖苷酶和酸性磷酸酶活性灰度值相较于非污染土壤,分别降低了5%和3%。【结论】青葙根际土壤酶活性的空间分布具有显著的异质性,与根系分布密切相关,且土壤镉污染显著抑制了β-葡萄糖苷酶和酸性磷酸酶的活性。[Objective]The aim was to investigate the influence of soil cadmium(Cd)contamination on the spatial distribution ofβ-glucosidase and acid phosphatase in the rhizosphere of Cd hyperaccumulator Celosia argentea Linn.[Method]The in-situ soil zymography was used to obtain the distribution ofβ-glucosidase and acid phosphatase in the rhizosphere of C.argentea in Cd-polluted soil and non-polluted soil.[Result]The Results showed that the hot spots ofβ-glucosidase and acid phosphatase were along entire root of C.argentea,which were relatively higher at the root tips.In both the polluted and non-polluted soils,the grey value ofβ-glucosidase and acid phosphatase at the root center were significantly higher than those 2 mm away from the root center.In Cd-polluted soil,the grey value ofβ-glucosidase and acid phosphatase in the root center decreased by 5%and 3%when compared to that in the non-polluted soil.[Conclusion]The results showed that the spatial distribution of soil enzyme activities was closely related to the root distribution.Soil Cd contamination had a significant negative influence on the soil enzyme activities.

关 键 词：土壤酶谱 青葙 镉污染 酶活性空间分布 

分 类 号：X53[环境科学与工程—环境工程]