EMS诱变的水稻不同位点突变体对ALS抑制剂类除草剂抗性差异  

Differences in ALS inhibitor herbicide resistance among rice mutants with different loci induced by EMS mutagenesis

作　　者：周佳伟 王歆凯 唐兆成 凌溪铁 张保龙 ZHOU Jiawei;WANG Xinkai;TANG Zhaocheng;LING Xitie;ZHANG Baolong(College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China;Institute of Germplasm Resources and Biotechnology,Jiangsu Academy of Agricultural Sciences/Jiangsu Provincial Key Laboratory of Agrobiology,Nanjing 210014,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室,江苏南京210014

出　　处：《江苏农业学报》2024年第7期1153-1160,共8页Jiangsu Journal of Agricultural Sciences

基　　金：“崖州湾”菁英人才科技专项(SCKJ-JYRC-2022-35);江苏省农业科技自主创新基金项目[CX(23)3094]

摘　　要：稻田杂草严重威胁水稻生长,培育抗除草剂水稻新品种,是高效解决稻田杂草危害的重要途径之一。本研究利用甲基磺酸乙酯(EMS)对华占水稻进行化学诱变,创建突变体,利用乙酰乳酸合成酶(ALS)抑制剂类除草剂甲咪唑烟酸(20 g/hm 2,有效成分)对突变体进行筛选,获得抗甲咪唑烟酸突变体材料,并对上述突变体的OsALS基因序列进行分析,明确其突变类型。最后对不同突变类型对甲氧咪草烟和甲基二磺隆的交互抗性进行分析。结果表明,华占水稻经EMS诱变和甲咪唑烟酸筛选后共获得11株抗甲咪唑烟酸突变体材料,其突变类型为Pro171Leu、Ser627Cys、Ser627Asn和Gly628Glu 4种。其中,Ser627Cys为首次发现的突变类型。4种突变类型对甲咪唑烟酸的抗性水平存在一定的差异,Ser627Asn、Ser627Cys、Pro171Leu、Gly628Glu突变类型的抗性指数分别为8.71、5.16、3.28和3.69。4种突变类型对甲氧咪草烟和甲基二磺隆的抗性亦存在显著差异。其中,Ser627Asn突变类型对甲氧咪草烟的抗性最高,抗性指数为20.57,Gly628Glu对甲基二磺隆抗性最高,抗性指数为5.11。综上,本研究通过EMS诱变获得了4种抗ALS抑制剂类除草剂的华占水稻突变类型,并鉴定了其对甲咪唑烟酸的抗性差异及对甲氧咪草烟和甲基二磺隆的交互抗性,为进一步的抗除草剂水稻品种培育和除草剂轮换使用提供依据。Weeds in paddy fields seriously threaten the growth of rice.Breeding new varieties of herbicide-resistant rice is one of the important ways to effectively solve the harm of weeds in paddy fields.In this study,ethyl methane sulfonate(EMS)was used as a chemical mutagen to induce Huazhan rice to create mutants.The mutants were screened by the acetolactate synthase(ALS)inhibitor herbicide imazapic(20 g/hm 2)to obtain resistant mutant materials.The OsALS gene sequences of the above mutants were analyzed to clarify the mutation type.Finally,the cross-resistance of different mutant types to imazamox and mesosulfuron-methyl was analyzed.The results showed that 11 imazapic-resistant mutants were obtained from Huazhan rice by EMS mutagenesis and imazapic screening.The mutation types were Pro171Leu,Ser627Cys,Ser627Asn and Gly628Glu.Among them,Ser627Cys mutation type was first reported.The resistance levels of the four mutation types to imazapic were different.The resistance indices of Ser627Asn,Ser627Cys,Pro171Leu and Gly628Glu were 8.71,5.16,3.28 and 3.69,respectively.There were also significant differences in the resistance of the four mutation types to imazamox and mesosulfuron-methyl.The resistance of Ser627Asn to imazamox was the highest,and the resistance index was 20.57.The resistance of Gly628Glu to mesosulfuron-methyl was the highest,and the resistance index was 5.11.In summary,four Huazhan rice mutant types resistant to ALS inhibitor herbicides were obtained by EMS mutagenesis,and their resistance differences to imazapic and the cross-resistance to imazamox and mesosulfuron-methyl were identified,which provided a basis for further cultivation of herbicide-resistant rice varieties and rotation of herbicides.

关 键 词：水稻 ALS抑制剂类除草剂 EMS诱变 抗除草剂突变体 

分 类 号：S511.035.2[农业科学—作物学]