稀土铽对斑马鱼急性毒性及抗氧化酶活性的影响  

Effects of terbium on acute toxicity and antioxidant enzyme activity of zebrafish

作　　者：曾露雪 边子俊 宁周神 陈明[1,2] 董伟[1,2,3] ZENG Luxue;BIAN Zijun;NING Zhoushen;CHEN Ming;DONG Wei(Jiangxi Key Laboratory of Mining and Metallurgy Environmental Pollution Control,Jiangxi University of Science and Technology,Ganzhou 341000,Jiangxi,China;School of Resources and Environmental Engineering,Jiangxi University of Science and Technology,Ganzhou 341000,Jiangxi,China;School of Life Sciences,Jiangxi University of Science and Technology,Ganzhou 341000,Jiangxi,China)

机构地区：[1]江西理工大学江西省矿冶环境污染控制重点实验室,江西赣州341000 [2]江西理工大学资源与环境工程学院,江西赣州341000 [3]江西理工大学生命科学学院,江西赣州341000

出　　处：《有色金属科学与工程》2024年第4期623-632,共10页Nonferrous Metals Science and Engineering

基　　金：国家重点研发计划资助项目(2019YFC1805100);江西省杰出青年基金资助项目(20212ACB213004);江西省青年井冈学者奖励计划资助项目(QNJG2020050);赣州市创新领军人才计划资助项目(202101095076)。

摘　　要：以斑马鱼(Danio rerio)为受试鱼种,采用半静态急性暴露实验,研究了稀土离子铽(Terbium,Tb)对斑马鱼的急性毒性,并根据急性毒性实验结果设置含Tb浓度分别为2.0、20.0、40.0 mg/L 3个组,分别对斑马鱼3个组织(头部、肌肉、内脏)的过氧化氢酶(CAT)活性和丙二醛(MDA)含量进行测定,以此来研究Tb对斑马鱼的毒理作用。结果表明,高浓度Tb会对斑马鱼产生较大的毒性,Tb对斑马鱼的48 h和96 h半致死浓度(LC50)分别为81.39 mg/L和79.03 mg/L,安全浓度(CS)为7.90 mg/L;Tb^(3+)对斑马鱼各部位CAT活性均有不同影响。随着Tb^(3+)浓度升高,CAT活力逐渐减弱,肌肉和内脏的CAT活性也随暴露时间的增加,在呈现前期被显著诱导后又被显著抑制,但40.0 mg/L浓度组内脏的CAT活力在净化期内始终未恢复。斑马鱼内脏MDA含量受Tb^(3+)胁迫,呈现诱导-抑制反复变化,肌肉MDA含量在胁迫期间表现为明显的诱导效应,在暴露的第14天时达到峰值为57.13%;40.0 mg/L浓度组内脏MDA含量在进入净化阶段仍被显著诱导,最高诱导率达28.99%。研究探究了Tb^(3+)对斑马鱼的急性毒性与氧化应激毒性,对防止和减少稀土离子对水生生物的毒性作用提供参考依据。The acute toxicity of Terbium(Tb)to zebrafish(Danio rerio)was investigated by a semi-static acute exposure test.Based on the acute toxicity test,the concentration groups of low(2.0 mg/L),medium(20.0 mg/L),and high(40.0 mg/L)of Tb were set up to determine the toxicological effects of Tb on zebrafish by analyzing catalase(CAT)activity and malondialdehyde(MDA)content in three tissues(head,muscle and viscera)of zebrafish.The results showed that a high concentration of Tb was more toxic to zebrafish.The 48 h and 96 h median lethal concentrations(LC50)of Tb to zebrafish were 81.39 mg/L and 79.03 mg/L,respectively.The safe concentration(CS)was 7.90 mg/L.Terbium(Ⅲ)ion(Tb^(3+))had different effects on CAT activity in various parts of zebrafish.CAT activity decreased gradually with the increase of Tb concentration.With increased exposure time in muscle and viscera,CAT activity was initially induced and then significantly inhibited,while it did not recover in viscera during the purification period in the 40mg/L group.Under Tb stress,the MDA content in viscera of zebrafish showed repeated induction-inhibition changes,while MDA content in muscle exhibited an obvious induction effect during stress,peaking 57.13%on the 14th day after exposure.The visceral MDA content of zebrafish treated with 40.0 mg/L concentration was still significantly induced at the purification phase,with the highest induction rate of 28.99%.The study revealed the acute toxicity and oxidative stress toxicity of Tb to zebrafish and provided a reference for preventing and reducing the toxicity of rare earth ions to aquatic organisms.

关 键 词：铽 斑马鱼 急性毒性 过氧化氢酶(CAT) 丙二醛(MDA) 

分 类 号：X503.225[环境科学与工程—环境工程]