血小板裂解物体外抑菌活性及其与抗生素的协同效应研究  

Antibacterial Effect of Platelet Lysate(PL)in Vitro and Its Synergistic Effect with Antibiotics

作　　者：冯慧慧 林丹 俞露 邓刚 FENG Huihui;LIN Dan;YU Lu;DENG Gang(Ningbo Central Blood Station,Ningbo 315010)

机构地区：[1]宁波市中心血站,浙江宁波315010

出　　处：《临床输血与检验》2024年第4期446-450,共5页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：宁波市卫生健康科技计划项目(No.2023Y35)资助。

摘　　要：目的通过体外实验探讨血小板裂解物(PL)的体外抑菌活性及其与抗生素的协同效应。方法选取大肠杆菌、肺炎克雷伯杆菌,采用微孔板法与牛津杯打孔法进行PL体外抑菌实验,微孔板法中设置庆大霉素(GM)、富血小板血浆(PRP)阳性对照,贫血小板血浆(PPP)阴性对照,测定OD值,计算抑菌率,牛津杯打孔法测定抑菌圈直径。结果对大肠杆菌的抑菌率GM>PL>PRP>PPP(P<0.05),且随着PL浓度的降低,抑菌率逐渐降低;PL对大肠杆菌的抑菌率在4小时达到高峰,随着时间的延长,抑菌率逐渐降低。PL与庆大霉素的联用增强了庆大霉素的抗菌作用,PL+GM抑菌圈直径>GM抑菌圈直径。PL对肺炎克雷伯杆菌的抑菌作用GM+PL>GM>PL。结论PL具有一定的抑菌作用,但弱于抗生素,其抑菌作用随着时间的延长及浓度的降低而逐渐减弱,并具有协同抗菌作用。Objective To investigate the antibacterial effects of platelet lysate(PL)in vitro and its synergistic effect with antibiotics.Methods Escherichia coli(E.coli)and K.pneumoniae were selected for PL in vitro antibacterial experiment by microplate method and Oxford cup drilling method.Gentamicin(GM),platelet rich plasma(PRP)positive control and platelet poor plasma(PPP)negative control was set in microplate method,OD value was determined,and antibacterial rate was calculated.The diameter of the antibacterial circle was determined by Oxford cup drilling method.Results The antibacterial rate against E.coli was found to be GM>PL>PRP>PPP(P<0.05),and the inhibition rate gradually decreased with the decrease of PL concentration;The antibacterial rate of PL on E.coli reached its peak at 4 hours,and gradually decreased with the prolongation of time.The combination of PL and GM enhanced the antibacterial effect of GM,with PL+GM zone of inhibition greater than GM zone of inhibition.The antibacterial effect against K.pneumoniae was found to be GM+PL>GM>PL.Conclusion PL has a certain antibacterial effect,but weaker than antibiotics.Its antibacterial effect gradually weakens with the prolongation of time and the decrease of concentration,and has a synergistic antibacterial effect.

关 键 词：富血小板血浆 血小板裂解物 抑菌实验 抑菌率 

分 类 号：R457[医药卫生—治疗学]