LED白光的亚甲蓝光化学法血浆病毒灭活研究  

Study of Viral Reduction by Methylene Blue with LED White Light in Plasma

作　　者：莫琴[1] 黄宇闻[1] 刘鸿 伍晓菲[1] 贾尧[1] 马荣钠 王迅[1] MO Qin;HUANG Yuwen;LIU Hong;WU Xiaofei;JIA Yao;MA Rongna;WANG Xun(Shanghai Blood Center,Shanghai 200051)

机构地区：[1]上海市血液中心,上海200051

出　　处：《临床输血与检验》2024年第4期463-469,共7页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：上海市卫生健康委员会卫生行业临床研究专项面上项目(No.202240210)资助。

摘　　要：目的探讨LED白光的亚甲蓝光化学法对血浆中的病毒灭活效果。方法血浆中加入终浓度为1μM的亚甲蓝,以LED白光为光源,比较不同处理方式(10 cm/5 cm照射间距、单侧/双侧照射)、不同照射强度(30000 lx、35000 lx、40000 lx、45000 lx、50000 lx)和不同处理时间点(10 min、20 min、30 min)的血浆中凝血因子的活性及Sindbis病毒的灭活效果。结果在50000 lx照射强度下,随着照射时间的增加,血浆温度逐渐升高,升温幅度为5 cm间距>10 cm间距,双侧照射>单侧照射。5 cm/10 cm间距、单侧/双侧照射对凝血因子Ⅷ和纤维蛋白原(FIB)活性的影响均没有显著差异。以10 cm间距、双侧照射的处理方式作为病毒灭活装置条件参数,用30000 lx、35000 lx、40000 lx、45000 lx和50000 lx的光照强度分别照射5 min后,血浆中Sindbis病毒被灭活,滴度下降均>4 LogTCID50/0.1 mL;照射20 min后,血浆中凝血因子Ⅷ活性>70%、FIB活性>60%。光照强度与凝血因子损失的相关分析表明:不同的光照强度与凝血因子Ⅷ的活性损失没有相关性,光照的强度对FIB的影响表现在照射的20 min以内,照射强度越大FIB活性下降越严重,但照射30 min后不同的光照强度对FIB的影响已没有显著性差异。照射时间与凝血因子的损失高度相关,不同光照强度下凝血因子都随着照射时间的增加而损失。结论LED白光可以作为亚甲蓝病毒灭活方法的新光源,在合适的条件下有效灭活血浆中的病毒,并保留血浆中凝血因子的活性。Objective To evaluate the effect of virus reduction in plasma using LED white light.Methods Methylene blue was added to plasma at a final concentration of 1μM.The activity of coagulation factors in plasma and the inactivation effect on Sindbis virus were compared by different treatment methods(10 cm/5 cm spacing,unilateral/bilateral irradiation),different light intensities(30000 lx,35000 lx,40000 lx,45000 lx,and 50000 lx)and different time points(10,20,and 30 min)using LED white light as the light source.Results Increase time of irradiation led to increase of plasma temperature under 50000 lx light intensity.The extent of increase was found to be greater at 5 cm than at 10 cm spacing,and greater with bilateral than unilateral irradiation.The 5 cm/10 cm spacing and unilateral/bilateral irradiation had no significant differences in their effect on coagulation factorⅧand fibrinogen(FIB)activity.The 10 cm bilateral spacing was selected as the device parameter for photochemical treatment.Plasma was irradiated at intensities of 30000,35000,40000,45000,and 50000 lx,respectively.The results showed Sindbis virus in plasma was inactivated after 5 minutes of irradiation at different light intensities,with titer reduction>4 LogTCID50/0.1 mL.FactorⅧand FIB activities were retained at the level of 70%and 60%,respectively after 20 minutes of irradiation.Correlation analysis revealed that there was no correlation between different light intensities and loss of factorⅧactivity.However,the effect of light intensities on FIB activity was more pronounced within 20 minutes of irradiation.A significant decline in the FIB activity was observed with increasing light intensities,although no significant difference was found after 30 minutes of irradiation.The irradiation time was found to be significantly correlated with the loss activity of coagulation factors,regardless of the light intensities.Conclusion LED white light can act as a new light source for methylene blue virus reduction.Under the right conditions it reduced viruses

关 键 词：LED白光 亚甲蓝 病毒灭活 

分 类 号：R457[医药卫生—治疗学]