纳米酶应用于抵抗红细胞氧化损伤可行性的初步研究  

Feasibility of Nanozymes Application Against Oxidative Damage of Red Blood Cells

作　　者：王雨婧 马海梅 WANG Yujing;MA Haimei(Department of Blood Transfusion,Beijing Tsinghua Changgung Hospital,School of Clinical Medicine,Tsinghua University,Beijing 102218)

机构地区：[1]清华大学附属北京清华长庚医院输血科,清华大学临床医学院,北京102218

出　　处：《临床输血与检验》2024年第4期484-490,共7页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的探究纳米酶在血液制品中的应用方向及可能性。方法通过水热法等方式合成具备应用潜质的无机纳米颗粒;利用透射电镜进行形态学表征;通过对过氧化氢及超氧阴离子的清除评估纳米粒子的抗氧化活性;通过光学显微镜以及光谱测定等方法分析纳米酶对血液中红细胞形态和功能的影响。结果通过探索实验合成了六种纳米粒子并进行了形态学表征。其中普鲁士蓝、空心普鲁士蓝、超小铱、氧化钌纳米粒子1 mg/mL浓度下具有大于75%的过氧化氢清除率;空心普鲁士蓝、普鲁士蓝氧化锰、超小铱、氧化钌纳米粒子1 mg/mL浓度下具有大于65%的超氧阴离子清除率。铱纳米酶和血红蛋白共孵育后,荧光光谱未发生波形改变。铱纳米酶和红细胞共孵育后,红细胞保持了良好的完整性。结论合成的六种纳米酶具有过氧化氢及超氧化物歧化酶活性,其中超小铱纳米酶粒径在1~2 nm,对血红蛋白结构及红细胞的完整性均没有影响,初步判断超小铱纳米酶具备应用于抵抗红细胞氧化损伤的潜能。Objective To explore the potential applications and possibility of nanozymes in reducing oxidative stress in blood products.Methods Inorganic nanoparticles with promising antioxidant properties were synthesized using the hydrothermal method.Morphological characterization was performed by transmission electron microscopy.By removing hydrogen peroxide and superoxide anion,we evaluated the antioxidant activities of the nanoparticles.The effects of these nanozymes on the morphology and function of red blood cells(RBCs)were analyzed via optical microscopy and spectroscopic techniques.Results Six distinct types of nanoparticles were synthesized and morphologically characterized,of which Prussian blue,hollow Prussian blue,ultra-small iridium,and ruthenium oxide nanoparticles exhibited more than 75%hydrogen peroxide clearance efficiency at a concentration of 1 mg/mL.Hollow Prussian blue,Prussian blue manganese oxide,ultrasmall iridium,and ruthenium oxide nanoparticles demonstrated a superoxide anion clearance rate greater than 65%at the same concentration.When incubated with iridium nanozyme and hemoglobin,the fluorescence spectra remained unchanged.RBCs maintained good structural integrity after co-incubation with iridium nanozyme.Conclusion The synthesized nanozymes have significant hydrogen peroxide and superoxide dismutase activities.Ultra-small iridium nanoparticle,with a size of 1-2 nm,do not compromise the structure of hemoglobin or RBC integrity.These findings preliminarily suggest that the ultra-small iridium nanozyme holds promise for application in combating oxidative damage of RBCs,thereby enhancing the safety and efficacy of blood products.

关 键 词：纳米酶 红细胞 抗氧化 可行性 

分 类 号：R457[医药卫生—治疗学]