单采血小板不同存储时间活化状态和微粒表达及个体差异分析  

Analysis of Activation State and Microparticle Level of Apheresis Platelets with Different Storage Time and Its Individual Differences

作　　者：王涛[1] 熊玉琪 黄瑶 王晓清[2] WANG Tao;XIONG Yuqi;HUANG Yao;WANG Xiaoqing(Testing of Donor Blood Center of Changzhou Central Blood Station;Department of Donor Service of Changzhou Central Blood Station,Changzhou,Jiangsu,213012)

机构地区：[1]常州市中心血站检测中心 [2]常州市中心血站献血服务科,江苏常州213012

出　　处：《临床输血与检验》2024年第5期597-603,共7页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的分析单采血小板保存时间对血小板活化的影响及献血者个体差异与采集后血小板活化状态的关系,为采供血机构血小板采集工作及临床个性化输注提供参考。方法随机收集2023年90例捐献者的单采血小板,随机保存1、2、3、4、7天,共5组。采用流式细胞仪检测各组血小板微粒(PMPs)、活化指标CD62p,进行统计学分析。收集各捐献者一般体检数据、捐献前血液检测数据和采集参数等数据,分析其与PMPs和血小板活化的关系。分析PMPs和CD62p的关系。结果储存1、2、3、4、7天的血小板CD62p表达率分别为(61.55±20.17)%、(69.05±13.09)%、(64.63±12.89)%、(70.34±9.504)%、(84.28±6.303)%;PMPs表达率分别为:(6.79±9.17)%、(7.01±9.13)%、(12.37±12.64)%、(7.51±5.70)%、(17.02±7.20)%;PMPs数量(×10^(3)/μL)分别为:2.69±4.84、4.80±9.20、3.59±3.38、7.94±8.89、145.1±190.3。单因素方差分析显示不同存储时长的各组单采血小板CD62p表达和PMPs表达存在显著差异(P<0.05)。各组两两比较,储存7天的血小板CD62p表达率和PMPs数量明显高于储存1、2、3、4天的血小板;其他各组间无明显差异。随储存时间的延长,血小板活化率和PMPs数量呈增长趋势。Pearson双变量分析显示,血小板采集后最初PMPs数量与捐献者脉搏和白细胞数目呈正相关(P<0.05),与其他因素无明显相关性;CD62p与PMPs的表达呈正相关(P<0.05)。结论单采血小板在保存4天时间内,活化和PMPs的表达无明显变化,之后出现活化和PMPs的表达增加的特点(P<0.05)。血小板采集后的活化与个体差异有关。可依据血小板储存过程中的活化和PMPs表达的特点选择相应产品应用临床。Objective To analyse the effect of apheresis platelets preservation time on platelet activation and the relationship between individual donor differences and platelet activation status after collection,so as to provide reference for the platelet collection work of blood collection and supply institutions and clinical personalized transfusion.Methods Apheresis platelets from 90 donors in 2023 were randomly collected and preserved for 1,2,3,4 and 7 days in 5 groups.Platelet microparticles(PMPs)and activation index CD62p were detected by flow cytometry in each group and statistically analysed.Data on general physical examination,pre-donation blood test data and collection parameters were collected from each donor and analysed for their relationship with PMPs and platelet activation.The relationship between PMPs and CD62p was analysed.Results The expression rates of CD62p in platelets stored for 1,2,3,4,and 7 days were(61.55±20.17)%,(69.05±13.09)%,(64.63±12.89)%,(70.34±9.504)%,and(84.28±6.303)%,respectively;and the expression rates of PMPs were(6.79±9.17)%,(7.01±9.13)%,(12.37±12.64)%,(7.51±5.70)%,and(17.02±7.20)%;and the number of PMPs(×10^(3)/μL)was 2.69±4.84,4.80±9.20,3.59±3.38,7.94±8.89,and 145.1±190.3,respectively.One-way ANOVA showed that Apheresis platelets from each group of different storage duration CD62p expression and PMPs expression were significantly different(P<0.05).Comparing each group,the CD62p expression rate and the number of PMPs in platelets stored for 7 days were significantly higher than those stored for 1,2,3,and 4 days;there was no significant difference between the other groups.Platelet activation rate and the number of PMPs tended to increase with storage time.Pearson's bivariate analysis showed that the number of PMPs initially after platelet collection was positively correlated with donor pulse and leukocyte counts(P<0.05),and there was no significant correlation with other factors;the expression of CD62p was positively correlated with the expression of PMPs(P<0.05).Concl

关 键 词：单采血小板 血小板微粒 血小板活化 血小板存储 个性化输注 

分 类 号：R457[医药卫生—治疗学]