基于蛋白组学和代谢组学在血液存储中相关性分析  

Integration of Proteomics and Metabolomics in Red Blood Cells Storage Lesion

作　　者：苏晓敏 张国权[1] 王锦[1] 徐华[1] SU Xiaomin;ZHANG Guoquan;Wang Jin;XU Hua(Shaanxi Blood Center,Xi'an,710061)

机构地区：[1]陕西省血液中心,西安710061

出　　处：《临床输血与检验》2024年第5期609-616,共8页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的红细胞(RBC)储存损伤一直是RBC体外储存研究所关注的重要问题,涉及到RBC储存过程中一系列生理、生化、形态和组学成分的改变,这些改变相互作用从而对储存RBC产生影响。为了进一步阐明RBC储存损伤机制,通过多组学研究储存期悬浮RBC损伤机制。方法取一次性使用塑料血袋采集的悬浮RBC共10袋(ALT化验结果不合格),将已制备的RBC放4℃冰箱中储存,分别于储存0周、1周、3周和5周取样,按RBC质量标准进行检测(HCT、HGB和储存期末溶血率)。检测其在不同保存期的各项生化指标(K^(+)、Na^(+)、Cl^(-)、Ca^(2+)、Glu和LDH)。进行蛋白组学和代谢组学检测,通过生物信息学分析评价悬浮RBC储存过程中相关生物学功能变化情况及相关性。结果符合悬浮RBC质量国家标准,其生化指标K^(+)和LDH随着保存时间的延长而有所升高,Na^(+)和Glu随着保存时间的延长而有所下降,在保存5周时差异显著(P<0.01),其它指标Cl^(-)和Ca^(2+)变化不明显(P>0.05)。我们根据筛查的蛋白质以及其下游的差异代谢物变化情况,进行了蛋白组学与代谢组学整合分析,通过不同层次的生物分子之间的关联研究,发现悬浮RBC的储存对RBC代谢等过程影响更明显,特别是核苷酸代谢、嘌呤代谢及蛋白质的消化和吸收等代谢相关通路,对RBC代谢相关蛋白影响反而小一些。结论我们通过使用代谢组学和蛋白组学检测,促进RBC结构、功能等的研究,为深入研究悬浮RBC储存损伤机制奠定了良好基础。Objective Red blood cells(RBCs)storage lesion has been an important concern on RBCs storage in vitro,involving a series of physiological,biochemical,morphological,and omics changes during RBCs storage.These changes interact with each other and affect stored RBCs.In order to further elucidate the mechanism of RBCs storage lesion,the mechanism of RBCs lesion during storage was conducted by multi-omics.Methods A total of 10 bags of RBCs were collected using disposable plastic blood bags(abnormal ALT test results).The prepared RBCs was stored at 4℃for 0,1,3 and 5 weeks,respectively,and tested according to RBC quality standards(HCT,HGB and hemolysis rate during storage).The biochemical indexes(K^(+)、Na^(+)、Cl^(-)、Ca^(2+)、Glu and LDH)were detected in different storage periods.Proteomic and metabolomic tests were performed,and bioinformatics analysis was used to evaluate the changes and correlation of biological functions during RBCs storage.Results In line with the national quality standard for RBCs,its biochemical indexes K^(+)and LDH increased with the preservation time,Na^(+)and Glu decreased.At 5 weeks,the difference was significant(P<0.01),and other indexes Cland Ca^(2+)had no significant changes(P>0.05).We performed proteomics and metabolomics based on the screened proteins and their downstream differential metabolite changes.By different levels of biomolecules,we found that RBCs storage had more effects on RBCs metabolism etc.,especially on metabolic pathways such as nucleotide metabolism,purine metabolism,and protein digestion and absorption,but had less effects on RBC metabolism-related proteins.Conclusion By metabolomics and proteomics,we promote the study of RBCs structure,function,etc.,which lays a good foundation for further research on the mechanism of RBCs storage lesion.

关 键 词：红细胞 代谢组学 蛋白组学 联合分析 

分 类 号：R457[医药卫生—治疗学]