加味曲直汤对膝关节骨性关节炎模型大鼠软骨及半月板损伤保护作用研究  

Approach to protective effect of modified Quzhi decoction on cartilage and meniscus injury in modeled rats with knee joint osteoarthritis

作　　者：周宇 陈炀 韩俊 黄海涛[2] 何伟东[2] 周中[2] ZHOU Yu;CHEN Yang;HAN Jun(Nanjing University of Traditional Chinese Medicine,Nanjing 210023,China;不详)

机构地区：[1]南京中医药大学,南京210023 [2]江苏省中西医结合医院

出　　处：《山西中医》2024年第9期58-62,共5页Shanxi Journal of Traditional Chinese Medicine

摘　　要：目的:探究加味曲直汤缓解膝关节骨性关节炎症状的可能作用机制。方法:将54只SPF级雄性大鼠分为空白组、模型组、加味曲直汤低剂量组、加味曲直汤中剂量组、加味曲直汤高剂量组、塞来昔布组,每组各9只,除空白组外其余各组采用碘乙酸钠关节内注射的方法制备膝骨关节炎大鼠模型,造模后药物干预6周。使用HE染色观察大鼠膝关节软骨及半月板病理形态改变,Mankin法评估软骨退变程度,ELISA检测大鼠血清中IL-1β、TNF-α、IL-17、IL-6、MMP-1的含量,免疫组化法检测半月板组织中IL-6、MMP-1表达情况,PCR法检测大鼠软骨中NF-κB、CIKS(NF-κB激活剂)mRNA表达水平。结果:与模型组比较,加味曲直汤组大鼠软骨结构稍有破坏,软骨表面欠光滑,软骨细胞分布不均,基质染色减少;半月板表面粗糙,个别半月板表面出现断裂、撕裂,细胞核分布不均匀、细胞核体积变大,出现局灶样的增多。加味曲直汤组大鼠血清中IL-1β、TNF-α、IL-17、IL-6、MMP-1表达水平较模型组均下降(P﹤0.05);免疫组化结果显示半月板组织中IL-6、MMP-1表达水平较模型组均下降(P﹤0.05)。与模型组比较,加味曲直汤组大鼠软骨中NF-κB、CIKS mRNA表达水平降低(P﹤0.05)。结论:加味曲直汤可以减轻KOA大鼠膝关节内的炎症反应,调节IL-17/NF-κB信号通路中相关分子的表达,缓解膝关节软骨及半月板的退变,达到改善膝关节骨性关节炎的效果。Objective:To approach the possible mechanism of modified Quzhi decoction on relieving the symptoms of knee joint osteoarthritis.Methods:54 SPF male rats were divided into blank group,model group,low-dose modified Quzhi decoction group,middle-dose modified Quzhi decoction group,high-dose modified Quzhi decoction group and celecoxib group,9 rats in each group.Except for the blank group,other groups were made into rat models of knee osteoarthritis by intra-articular injection of sodium iodoacetate,and the drug intervention was conducted for 6 weeks after modeling.The pathomorphological changes of cartilage and meniscus of knee joint in rats were observed by HE staining.The degree of cartilage degeneration was evaluated by Mankin method.The contents of IL-1β,TNF-α,IL-17,IL-6 and MMP-1 in rat's serum were detected by ELISA.The expressions of IL-6 and MMP-1 in meniscus tissue were detected by immunohistochemistry.The expression levels of NF-κB and CIKS(NF-κB activator)mRNA in rat's cartilage were detected by PCR method.Results:Compared with the model group,the structure of rat's cartilage in modified Quzhi decoction group was slightly damaged,the surface of cartilage was undersmooth,the distribution of chondrocytes was uneven,and the matrix staining was reduced;the surface of meniscus was rough,the surfaces of some meniscus were broken and torn,the distribution of nuclei was uneven,the volume of nuclei became larger,the focal appearance increased.The expression levels of IL-1β,TNF-α,IL-17,IL-6 and MMP-1 in serum of rats in modified Quzhi decoction group were lower than those in model group(P﹤0.05).The results of immunohistochemistry showed that the expression levels of IL-6 and MMP-1 in meniscus tissue were lower than those in model group(P﹤0.05).Compared with the model group,the expression levels of NF-κB and CIKSmRNA in cartilage of rats in modified Quzhi decoction group were decreased(P﹤0.05).Conclusion:Modified Quzhi decoction can alleviate the inflammatory reaction in knee joint of KOA rats,regulate t

关 键 词：膝关节骨性关节炎 加味曲直汤 实验研究 

分 类 号：R684.3[医药卫生—骨科学]