凝胶色谱法进行聚乙烯吡咯烷酮的含量测定  

Determination of Polyvinylpyrrolidone by Gel Chromatography

作　　者：朱广东 王宇 乐文伟 Zhu Guangdong;Wang Yu;Yue Wenwei(Shanghai Yuking Water Soluble Material Technology Corporation Limited,Shanghai 200120,China)

机构地区：[1]上海宇昂水性新材料科技股份有限公司,上海200120

出　　处：《化学世界》2024年第5期318-321,共4页Chemical World

摘　　要：建立了凝胶色谱-紫外法测定聚乙烯吡咯烷酮含量的方法,并经方法学验证其具有良好的专属可行性。以0.1 mol/L氯化钠/乙腈(7∶3,V∶V)作为流动相,采用聚合物基质凝胶色谱柱,检测波长205 nm。聚乙烯吡咯烷酮的质量浓度在0.1~1.5 mg/mL范围内与色谱峰面积呈良好的线性关系,线性相关系数为0.9999。聚乙烯吡咯烷酮的高、中、低3种质量浓度的回收率为99.3%~102.0%,相对标准偏差(RSD)为0.35%~0.81%,符合测定要求,准确度良好。A gel chromatography-ultraviolet method for determining polyvinylpyrrolidone content was established,and it was proved to possess good exclusive feasibility.The detection wavelength is 205 nm on a polymer gel column with 0.1 mol/L sodium chloride as mobile phase and acetonitrile(7∶3,V∶V)as mobile phase.The mass concentration of polyvinylpyrrolidone shows a good linear relationship with peak area in the range of 0.1~1.5 mg/mL,and the linear correlation coefficient is0.9999.The recoveries are 99.3%~102.0%and relative standard deviation(RSD)is 0.35%~0.81%at high,medium and low concentrations,which can meet the determination requirements with good accuracy.This established gel chromatography method is accurate,simple and suitable for the determination of polyvinylpyrrolidone in different applications.

关 键 词：聚乙烯吡咯烷酮 凝胶色谱 紫外 含量测定 

分 类 号：O657.7[理学—分析化学]