红花对系统性硬化症小鼠皮肤及肺组织IL-33、ST2的影响  

Effects of Honghua on IL-33 and ST2 in skin and lung tissues of mice with systemic sclerosis

作　　者：张佳佳 黄奕[1] 李凯[2] 吕军影[2] Zhang Jiajia;Huang Yi;Li Kai;Lyu Junying(First Clinical Medical School,Guangxi Medical University,Nanning Guangxi 530021;First Affiliated Hospital,Guangxi Medical University,Nanning Guangxi 530021)

机构地区：[1]广西医科大学第一临床医学院,广西南宁530021 [2]广西医科大学第一附属医院,广西南宁530021

出　　处：《山西中医药大学学报》2024年第8期865-872,共8页Journal of Shanxi University of Chinese Medicine

基　　金：广西自然科学基金重点项目(2018GXNSFDA281047);广西中医药重点学科建设项目(GZXK-Z-20-52)。

摘　　要：目的:观察红花对博来霉素(BLM)诱导的系统性硬化症(SSc)小鼠皮肤和肺组织IL-33、ST2表达水平的影响,探究红花减轻SSc小鼠皮肤及肺组织纤维化的作用机制。方法:将32只BALB/C小鼠随机分为醋酸泼尼松组、红花水煎液组、模型组、对照组,每组8只。除对照组外,其他各组小鼠背部皮下注射BLM诱导建立SSc小鼠模型,0.1 ml/次,1次/d,连续28 d;造模期间,醋酸泼尼松组小鼠灌胃浓度为0.45 g/ml的醋酸泼尼松溶液0.2 ml/次,1次/d,连续28 d;红花水煎液组小鼠灌胃浓度为0.15 g/ml的红花水煎液0.2 ml/次,1次/d,连续28 d;对照组和模型组小鼠灌胃生理盐水0.2 ml/次,1次/d,连续28 d。给药期间观察各组小鼠一般生存状态及皮肤硬化情况,给药结束后禁食12 h,予腹腔注射2%戊巴比妥钠过量麻醉处死小鼠后取材。小鼠皮肤和肺组织切片脱蜡后行HE、Masson染色,根据皮肤HE玻片白细胞计数评定皮肤炎症水平,根据皮肤Masson染色胶原容积分数(CVF)评估皮肤的纤维化程度;分别采用Szapiel、Ashcroft评分系统评价小鼠肺部炎症水平及纤维化水平;免疫组织化学法(IHC)检测小鼠皮肤和肺组织的IL-33、ST2表达。结果:(1)与模型组小鼠比较,红花水煎液组和醋酸泼尼松组小鼠的精神状态、体质量、反应水平、背部剃毛区域被毛再生情况、皮肤弹性及硬度等有不同程度的改善。(2)对照组小鼠皮肤和肺组织形态结构基本正常,局部可见少量炎症反应;模型组小鼠皮肤和肺组织可见密集炎症细胞浸润和胶原纤维大量沉积;红花水煎液组和醋酸泼尼松组小鼠皮肤和肺组织炎症细胞浸润和胶原纤维沉积较模型组有所减轻。模型组小鼠皮肤炎症评分、CVF及肺组织Ashcroft、Szapiel评分较对照组明显升高,差异有统计学意义(P<0.01);红花水煎液组和醋酸泼尼松组小鼠上述指标较模型组明显下降,差异有统计学意义(P<0.01)。(3)与对照组比较,模�Objective:To observe the effects of Honghua on the expression levels of IL-33 and ST2 in skin and lung tissues of mice with bleomycin(BLM)-induced systemic sclerosis(SSc),and to explore the mechanism by which Honghua alleviates fibrosis in the skin and lung tissues of SSc mice.Methods:Thirty-two BALB/C mice were randomly divided into four groups:dexamethasone acetate group,Honghua decoction group,model group,and control group,with eight mice in each group.BLM was subcutaneously injected into the back of the mice to establish a mouse model of SSc at a dose of 0.1 ml per injection,once daily for 28 days.During the modeling period,the dexamethasone acetate group received 0.2 ml of a 0.45 g/ml dexamethasone acetate solution by oral gavage,once daily for 28 days.Honghua decoction group received 0.2 ml of a 0.15 g/ml Honghua Decoction by oral gavage,once daily for 28 days.The control and model groups received 0.2 ml of saline by oral gavage,once daily for 28 days.General survival status and skin hardening were observed during the treatment period.After 12 hours of fasting,the mice were euthanized with an overdose of 2%sodium pentobarbital and tissues were collected.Skin and lung tissue sections were stained with HE and Masson′s trichrome.Skin inflammation was evaluated based on white blood cell counts in HE-stained slides,and skin fibrosis was assessed by collagen volume fraction(CVF)in Masson′s trichrome-stained slides.Szapiel and Ashcroft scoring systems were used to evaluate lung inflammation and fibrosis levels.Immunohistochemistry(IHC)was performed to detect the expression of IL-33 and ST2 in skin and lung tissues.Results:(1)Compared with the model group,Honghua decoction and dexamethasone acetate groups showed improved general conditions,including mental state,body weight,response level,hair regrowth in shaved areas,skin elasticity,and hardness.(2)The control group displayed normal morphology of skin and lung tissues with minor inflammation.The model group showed dense infiltration of inflammatory cells and e

关 键 词：SSC 红花水煎液 白介素-33 肿瘤发生抑制蛋白2 实验研究 

分 类 号：R285.5[医药卫生—中药学]