两种棉花耐盐鉴定方法的综合评价与低酚棉种质系的耐盐性鉴定  

Comprehensive evaluation of two salt tolerance identification methods and salt tolerance identification of glandless cotton germplasms

作　　者：李昊旻 尹红 薛龙朔 张劭文 石钇琦 马心雨 赵天伦 陈进红[1] 祝水金[1] Li Haomin;Yin Hong;Xue Longshuo;Zhang Shaowen;Shi Yiqi;Ma Xinyu;Zhao Tianlun;Chen Jinhong;Zhu Shuijin(College of Agriculture and Biotechnology of Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学农业与生物技术学院,杭州310058

出　　处：《棉花学报》2024年第4期306-319,共14页Cotton Science

基　　金：科技创新2030重大项目(2023ZD0403803)。

摘　　要：【目的】探究陆地棉对人工海水和NaCl单盐胁迫的响应,评估人工海水模拟盐胁迫鉴定棉花耐盐性的可行性。【方法】以135份不同类型的陆地棉种质系为材料,比较人工海水和NaCl单盐胁迫对棉花种子萌发和幼苗生长的影响,结合主成分分析、隶属函数分析和聚类分析等方法对棉花材料的耐盐性进行综合评价,并通过田间试验对2种鉴定方法进行验证。【结果】2种盐胁迫下的鉴定结果仅有52.38%的一致性,鉴定结果存在较大差异。人工海水胁迫下的鉴定结果与田间试验鉴定结果呈极显著正相关关系,相关系数为0.720,NaCl胁迫下的鉴定结果与大田鉴定结果无显著相关关系。人工海水和NaCl胁迫处理下,105份低酚棉种质资源中表现为抗盐或耐盐的材料数量占比分别为21.90%和33.33%,其中鲁17和邯低酚29在2种胁迫下均表现出较强的耐盐性。【结论】使用与海涂地成分相似的人工海水模拟盐胁迫可以更加精确地鉴定棉花材料的耐盐性。低酚棉整体耐盐性较差,但不乏有耐盐性强的种质系,可用于培育耐盐的低酚棉新品种。[Objective]This study aims to explore the response of Gossypium hirsutum under artificial seawater and NaCl stresses,and to assess the feasibility of using artificial seawater to simulate salt stress for evaluating salt tolerance of cotton germplasms.[Methods]Utilizing 135 distinct G.hirsutum germplasms as the experimental materials,this study investigated the impact of artificial seawater and NaCl stresses on cotton seed germination and seedling growth.Through the integration of principal component analysis,membership function analysis,and cluster analysis methodologies,the comprehensive evaluation of cotton salt tolerance was conducted.The results of two identification methods were verified by field experiments under natural salt stress.[Results]The consistency of the identification results under the two salt stresses were only 52.38%,and there were great differences in the results.The identification results under artificial seawater stress were significantly and positively correlated with the results of field experiments,with a correlation coefficient of 0.720;while that under NaCl stress were not significantly correlated with the field identification results.Under artificial seawater and NaCl stress treatments,21.90%and 33.33%of the 105 glandless cotton germplasms were resistant or tolerant to salt stress,respectively.Among them,Lu 17 and Handifen 29 showed strong salt tolerance under the two salt treatments.[Conclusion]Using artificial seawater that simulates the composition of coastal soil can identify the salt tolerance of cotton germplasms more accurately.Glandless cotton generally exhibits poorer salt tolerance,but there are still some germplasms with strong salt tolerance that can be used to breed new salt tolerant glandless cotton cultivars.

关 键 词：低酚棉 萌发 苗期 人工海水 耐盐性鉴定 盐胁迫 

分 类 号：S562[农业科学—作物学]