蓝莓组培中的继代培养技术研究  

Study on Subculture Technology of Blueberry Tissue Culture

作　　者：朱文菁 邓素珍 陈腾坚 吴志佳 孙泽 张嘉雄 陆胜林 郭艺鹏 ZHU Wen-jing;DENG Su-zhen;CHEN Teng-jian;WU Zhi-jia;SUN Ze;ZHANG Jia-xiong;LU Sheng-in;GUO Yi-peng(Yulin Normal University,Yulin 537000,Guangxi,China)

机构地区：[1]玉林师范学院,广西玉林537000

出　　处：《南方园艺》2024年第4期8-14,共7页Southern Horticulture

基　　金：玉林市科技计划项目(玉市科202235130);大学生创新创业训练计划项目(202110606165);玉林师范学院玉林师范学院博士启动项目(G2019ZK34)。

摘　　要：蓝莓是一种新型的保健果品,已被越来越多的国家所重视。以蓝莓当年生带腋芽茎段为组培外植体材料,搭配IAA、CPPU、ZT等激素,探讨其初代培养过程中消毒时间和不同激素浓度对继代培养适宜的培养基组合。试验结果表明:最佳消毒时间为75%酒精处理5min与0.1%升汞处理6min,成活率达90%;最佳愈伤增殖培养基为WPM+TDZ 0.05mg/L+IAA0.1mg/L,不定芽分化率达100%;最佳继代培养基为WPM+IAA0.1mg/L+ZT1mg/L株高为2.73.cm,植株生长健壮,增殖系数为5.6。Blueberry is a new kind of health fruit,which has been paid more and more attention in many countries.The suitable medium combination of disinfection time and different hormone concentrations for subculture of blueberry in the course of primary culture was studied by using the stem segment with axillary buds as explants with IAA,CPPU,ZT and other hormones.The results showed that the best disinfection time was 5 min with 75%alcohol and 6 min with 0.1%Hg and the survival rate reached 90%.The optimal medium for callus proliferation was WPM+TDZ 0.05 mg/L+IAA 0.1 mg/L,and the adventitious bud differentiation rate reached 100%.The optimal subculture medium was WPM+IAA 0.1 mg/L+ZT 1 mg/L,the average plant height was 2.73 cm,the growth was strong and the proliferation coefficient was 5.6.This study provides experimental basis for the rapid propagation and promotion of blueberry production,and has certain research significance.

关 键 词：蓝莓 茎段 组培 继代培养 

分 类 号：S663[农业科学—果树学]