骨痿愈方通过调节OPG/RANKL治疗绝经后骨质疏松的动物实验及机制研究  

Animal experiment and mechanism study of Guweiyu decoction for treating postmenopausal osteoporosis by regulating OPG/RANKL pathway

作　　者：扶浪 皮超 罗焘 伍光辉 徐祖健 陈孝均 马文哲 罗琳 FU Lang;PI Chao;LUO Tao;WU Guanghui;XU Zujian;CHEN Xiaojun;MA Wenzhe;LUO Lin(Department of Trauma,Luzhou Hospital of Traditional Chinese Medicine,Luzhou 646000,China;School of Pharmacy,South-west Medical University,Luzhou 646000,China;Department of Orthopaedics,The Affiliated Hospital of Traditional Chinese Medicine,Southwest Medical University,Luzhou 646000,China;State Key Laboratory of Quality Research of Traditional Chinese Medicine,Ma-cao University of Science and Technology,Macao 999078,China)

机构地区：[1]泸州市中医医院创伤科,泸州646000 [2]西南医科大学药学院,泸州646000 [3]西南医科大学附属中医医院骨科,泸州646000 [4]澳门科技大学中药质量研究国家重点实验室,中国澳门999078

出　　处：《西南医科大学学报》2024年第5期442-446,共5页Journal of Southwest Medical University

基　　金：四川省科技厅计划项目(2020YJ0373);西南医科大学-西南医科大学附属中医医院联合课题(2020XYLH-051)。

摘　　要：目的通过动物实验探究骨痿愈方治疗绝经后骨质疏松的有效性及其作用机制。方法将40只12周龄无特定病原体(specific-pathogen Free,SPF)级雌性SD大鼠随机分为假手术组(Sham)、模型组(OVX)、对照组(AS)、骨痿愈方组(GWY)。构建去卵巢骨质疏松症模型,骨痿愈方组予以骨痿愈方浓缩液灌胃;对照组予以阿仑膦酸钠灌胃;假手术组和模型组予以生理盐水灌胃。8周后处死动物,检测各组血清中钙、磷及骨源性碱性磷酸酶(bone alkaline phosphatase,BALP)、OPG、RANKL含量;Micro-CT检测各组L3椎体以评估骨密度变化。结果①血清检测:4组中Ca、P、BALP、OPG、RANKL及OPG/RANKL差异均具有统计学意义(P<0.05)。与Sham组比较,OVX组、AS组和GWY组Ca、P、BALP降低(P<0.05),OVX组OPG降低(P<0.05),RANKL升高(P<0.05),OPG/RANKL降低(P<0.05);与OVX组比较,AS组和GWY组Ca、P、BALP增加(P<0.05),AS组和GWY组OPG升高(P<0.05),RANKL降低(P<0.05),OPG/RANKL升高(P<0.05);②骨密度:各组BMD、BV/TV、Tb.Th、Tb.N、Tb.Sp差异均具有统计学意义(P<0.05)。与Sham组相比,AS组、GWY组腰椎BMD、BV/TV、Tb.Th、Tb.N显著降低(P<0.05),Tb.Sp提高(P<0.05)。与OVX组相比较,GWY组、AS组腰椎BMD、BV/TV、Tb.Th、Tb.N增加(P<0.05),Tb.Sp下降(P<0.05)。结论骨痿愈方可通过调控OPG/RANKL信号通路治疗绝经后骨质疏松。Objective To investigate the effectiveness and mechanism of Guweiyu decoction in treating postmenopausal osteopo-rosis through animal experiments.Methods The 12-week-old female SD rats with specific pathogen-free(SPF)status were randomly divided into the sham operation group,model group,control group,and Guweiyu decoction group.A osteoporosis model was constructed,and the Guweiyu decoction group was given a concatenated Guweiyu decoction gavage;the control group was given sodium alendronate gavage;and the sham operation group and model group were given physiological saline gavage.After 8 weeks,the animals were sacri-ficed,and the serum levels of calcium,phosphorus,and bone alkaline phosphatase(BALP)were measured in each group.Micro-CT was used to evaluate the bone density changes in the L3 vertebrae of each group.Results①Serum detection:There were significant sta-tistical differences in the content of Ca,P,BALP,OPG,RANKL,and OPG/RANKL among the four groups(P<0.05).Compared with the Sham group,the content of Ca,P,and BALP in the OVX group,AS group,and GWY group decreased(P<0.05),and OPG content in the OVX group decreased(P<0.05),RANKL content increased(P<0.05),and OPG/RANKL content decreased(P<0.05);com-pared with the OVX group,the content of Ca,P,and BALP in the AS group and GWY group increased(P<0.05),OPG content in the AS group and GWY group increased(P<0.05),RANKL content decreased(P<0.05),and OPG/RANKL content increased(P<0.05).②Bone density:The BMD,BV/TV,Tb.Th,Tb.N,and Tb.Sp of each group had significant statistical differences(P<0.05).Com-pared with the Sham group,the lumbar BMD,BV/TV,Tb.Th,and Tb.N of the AS group and GWY group significantly decreased(P<0.05),and Tb.Sp improved(P<0.05).Compared with the OVX group,the lumbar BMD,BV/TV,Tb.Th,and Tb.N of the GWY group and AS group increased(P<0.05),and Tb.Sp decreased(P<0.05).Conclusion Guweiyu decoction might be able to treat postmeno-pausal osteoporosis by regulating OPG/RANKL signaling pathway.

关 键 词：OPG/RANKL信号通路 骨痿愈方 绝经后骨质疏松 骨代谢 骨密度 

分 类 号：R285.5[医药卫生—中药学]