解淀粉芽孢杆菌YN201728对烟草白粉病的盆栽防效及对防御酶活性的影响  

Control effect on tobacco powdery mildew in pot experiment and influences on activities of defense enzymes of Bacillus amyloliquefaciens YN201728

作　　者：焦蓉 刘迎龙 赵静[1] 刘科新 王军伟 徐荣 JIAO Rong;LIU Yinglong;ZHAO Jing;LIU Kexin;WANG Junwei;XU Rong(Yuxi Normal University,Yuxi 653100,Yunnan,China;Yunnan Agricultural University,Kunming 650201,China;Yunnan Nanbao Biotechnology Co.,Ltd.,Yuxi 653100,Yunnan,China;Hongta Tobacco(Group)Co.,Ltd.,Yuxi 653100,Yunnan,China)

机构地区：[1]玉溪师范学院,云南省玉溪市红塔区653100 [2]云南农业大学,昆明市盘龙区650201 [3]云南南宝生物科技有限责任公司,云南省玉溪市红塔区653100 [4]红塔烟草(集团)有限责任公司,云南省玉溪市653100

出　　处：《烟草科技》2024年第8期58-66,共9页Tobacco Science & Technology

基　　金：国家自然科学基金项目“对羟基苯甲酸化感促进尖孢镰刀菌致病性作用机制研究”(32060036);云南省科技厅科技计划项目“灯盏花抗根腐病有益内生菌的筛选及绿色防控技术研究”(202101BA070001-024)。

摘　　要：为探究解淀粉芽孢杆菌(Bacillus amyloliquefaciens)YN201728对烟草白粉病的防效,明确YN201728对烟草白粉病病原菌(二孢白粉菌,Erysiphe cichoracearum)侵染后烟叶中防御酶[过氧化物酶(Peroxidase,POD)、苯丙氨酸解氨酶(Phenylalnine ammonialyase,PAL)、多酚氧化酶(Polyphenol oxidase,PPO)]活性的影响,通过盆栽试验分析YN201728发酵液、发酵滤液、菌体沉淀重悬液、菌体细胞破碎液对烟草白粉病的防治效果,采用水琼脂玻片法分析发酵滤液对二孢白粉菌孢子萌发的抑制效果,并通过盆栽试验分析YN201728发酵液和二孢白粉菌孢子共接种处理后烟叶中POD、PAL、PPO活性的变化。结果表明,盆栽条件下YN201728发酵液处理与发酵滤液处理对烟草白粉病的相对防效无显著差异,发酵液与发酵滤液喷施后7 d相对防效分别为61.64%和56.95%,菌体沉淀重悬液处理和菌体细胞破碎液处理最高相对防效分别为18.70%和10.80%;发酵滤液对二孢白粉菌孢子萌发有显著的抑制效果,培养基中发酵滤液含量(体积分数)为20.0%时,二孢白粉菌孢子萌发抑制率为92.07%。受到二孢白粉菌侵染时,YN201728发酵液处理的烟叶中POD和PAL活性未显著高于无菌水处理,该菌株未通过诱导POD或PAL防御酶活性提高增强植株对烟草白粉病的抗性。To investigate the control effect of Bacillus amyloliquefaciens YN201728 on tobacco powdery mildew,and to clarify the influences of YN201728 on the activities of defense enzymes(peroxidase[POD],phenylalnine ammonialyase[PAL]and polyphenol oxidase[PPO])in tobacco leaves infected by pathogen of tobacco powdery mildew(Erysiphe cichoracearum),pot experiments were conducted to analyze the control effects of YN201728 fermentation broth,fermentation filtrate,bacterial cell precipitation resuspension,bacterial cell crushing liquid on tobacco powdery mildew.The water agar slide method was used to investigate the inhibitory effect of the fermentation filtrate on spore germination of Erysiphe cichoracearum,and the changes in the activities of POD,PAL and PPO in tobacco leaves inoculated with YN201728 fermentation broth and Erysiphe cichoracearum were analyzed through pot experiments.The results showed that there was no significant difference in the relative control efficacies on tobacco powdery mildew between fermentation broth treatment and the fermentation filtrate treatment under pot conditions,and the control efficacies reached 61.64%and 56.95%respectively after 7-day treatments,and the highest relative control efficacies of the treatments of bacterial cell precipitation resuspension and bacterial cell crushing liquid were 18.70%and 10.80%,respectively.Tested fermentation filtrate had a significant inhibitory effect on spore germination,and the inhibition rate of the fermentation filtrate with the content(volume fraction)of 20.0%on spore germination of Erysiphe cichoracearum was 92.07%.When tobacco was infected with Erysiphe cichoracearum,the activities of POD and PAL in tobacco leaves treated with the fermentation broth were not significantly higher than those treated with aseptic water,the strain did not improve the resistance to tobacco powdery mildew by inducing increases in the activities of POD and PAL defense enzyme.

关 键 词：解淀粉芽孢杆菌 烟草白粉病 防治效果 防御酶 

分 类 号：S476[农业科学—农业昆虫与害虫防治]