谷胱甘肽引发对老化燕麦种胚线粒体抗氧化特性的影响  

Effects of glutathione priming on the mitochondrial antioxidant characteristics of aged oat seed embryos

作　　者：米春娇 洪流 马馼 毛培胜[1] MI Chun-jiao;HONG Liu;MA Wen;MAO Pei-sheng(College of Grassland Science and Technology,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学草业科学与技术学院,北京100193

出　　处：《草业学报》2024年第9期51-59,共9页Acta Prataculturae Sinica

基　　金：青藏高原主栽牧草种子包衣技术研发(SJCZFY2022-10);现代农业产业技术体系项目(CARS-34)资助。

摘　　要：本研究探讨了谷胱甘肽(glutathione,GSH)引发对老化燕麦种胚线粒体抗氧化能力的影响,以期为种子合理贮藏与利用提供科学依据。试验以老化5 d种子为材料,研究了0.2 mmol·L^(-1) GSH引发24 h处理下种胚线粒体抗氧化酶活性、抗氧化物和过氧化氢(H_(2)O_(2))含量的变化规律。结果表明,种子老化导致种胚线粒体内H_(2)O_(2)含量显著(P<0.05)升高,过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、谷胱甘肽还原酶(GR)和脱氢抗坏血酸还原酶(DHAR)活性均显著(P<0.05)降低,抗坏血酸(AsA)含量无显著变化,GSH含量、AsA/脱氢抗坏血酸(DHA)和GSH/氧化型谷胱甘肽(GSSG)显著(P<0.05)升高,DHA和GSSG含量显著(P<0.05)下降。而GSH引发处理使老化种子胚中线粒体的H_(2)O_(2)含量显著(P<0.05)降低,CAT和GR活性显著(P<0.05)升高,POD、SOD、MDHAR、APX、DHAR活性以及AsA、DHA含量和GSH/GSSG有所增加,GSH、GSSG含量和AsA/DHA有所降低,但差异均不显著。综上,GSH引发处理主要通过提高老化燕麦种胚线粒体抗氧化酶活性,降低H_(2)O_(2)含量,有效缓解了氧化损伤,维持种子活力。This study investigated the effects of glutathione(GSH)priming on the antioxidant capacity of aged oat(Avena sativa)seed embryo mitochondria,aiming to provide scientific evidence for the improving outcomes of storage and utilization of seeds.Using 5-day-old aged seeds,this experiment examined the variations in antioxidant enzyme activity,and antioxidant and hydrogen peroxide(H_(2)O_(2))contents in embryo mitochondria treated by priming with 0.2 mmol·L^(-1) GSH for 24 hours.The results indicated that seed aging significantly(P<0.05)increased the H_(2)O_(2) content in the embryo mitochondria and significantly(P<0.05)decreased the activities of antioxidant enzymes catalase(CAT),peroxidase(POD),superoxide dismutase(SOD),ascorbate peroxidase(APX),monodehydroascorbate reductase(MDHAR),glutathione reductase(GR),and dehydroascorbate reductase(DHAR).The content of ascorbic acid(AsA)showed no significant change,while GSH content,AsA/dehydroascorbic acid(DHA)ratio,and GSH/oxidized glutathione(GSSG)ratio significantly(P<0.05)increased,and DHA and GSSG contents significantly(P<0.05)decreased.Moreover,GSH priming treatment in aged seeds significantly(P<0.05)decreased H_(2)O_(2) content and significantly(P<0.05)increased the activities of CAT and GR in the embryo mitochondria.POD,SOD,MDHAR,APX,DHAR activities,AsA and DHA contents and GSH/GSSG ratio showed an increase,GSH and GSSG contents and AsA/DHA ratio showed a decrease,but none of these differences were significant.In conclusion,GSH priming treatment effectively mitigated oxidative damage and maintained seed vigor mainly by enhancing mitochondrial antioxidant enzyme activities and reducing H_(2)O_(2) content in aged oat seed embryos.

关 键 词：GSH引发 种子老化 燕麦 抗氧化能力 种子活力 

分 类 号：S544.9[农业科学—作物学]