蒺藜苜蓿OSCA基因家族鉴定及低温逆境表达分析  

Genome-wide analysis and expression of the OSCA family genes from Medicago truncatula in response to low temperature stresses

作　　者：崔红丽 孙明哲[1] 贾博为 孙晓丽[1] CUI Hong-li;SUN Ming-zhe;JIA Bo-wei;SUN Xiao-li(Crop Stress Molecular Biology Laboratory,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Key Laboratory of Germplasm Enhancement,Physiology and Ecology of Food Crops in Cold Region,Ministry of Education,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]黑龙江八一农垦大学作物逆境分子生物学实验室,黑龙江大庆163319 [2]东北农业大学寒地粮食作物种质创新与生理生态教育部重点实验室,黑龙江哈尔滨150030

出　　处：《草业学报》2024年第9期111-125,共15页Acta Prataculturae Sinica

基　　金：科技创新2030-重大项目(2022ZD04012);国家自然科学基金(32000212,32101672);黑龙江省自然科学基金(YQ2023C035);黑龙江省“双一流”学科协同创新成果项目(LJGXCG2023-072);黑龙江八一农垦大学研究生创新科研项目(YJSCX2022-Y02)资助。

摘　　要：高渗门控钙渗透通道(hyperosmolality-gated calcium-permeable channel,OSCA)是一类高渗胁迫感受器。本研究通过全基因组鉴定,从蒺藜苜蓿中筛选鉴定出13个MtOSCAs基因,并根据其与拟南芥OSCA基因家族成员的同源性命名为MtOSCA1.1~4.1。染色体定位分析表明MtOSCAs基因不均匀地分布在8条染色体上。MtOSCA家族按系统进化关系可分为4个亚家族,且同一亚家族内的成员具有相似的内含子-外显子模式。功能结构域和保守基序分析表明该家族在进化中高度保守,进一步共线性分析发现MtOSCAs和大豆OSCAs亲缘关系近,而与拟南芥OSCAs亲缘关系远。基因表达模式分析发现,不同亚家族的MtOSCAs基因表现出组织特异性。非生物胁迫转录组数据分析及qRT-PCR分析表明MtOSCA2.5/2.6/3.1显著受低温胁迫诱导表达。顺式作用元件分析证实MtOSCAs启动子区包含多种光、激素和逆境响应的顺式作用元件。上述结果为今后MtOSCAs基因调控蒺藜苜蓿抗逆性的功能研究奠定了坚实的理论基础。Hyperosmolality-gated calcium-permeable channel(OSCA)is a class of hyperosmotic sensors.A total of 13 MtOSCAs genes were identified by searching the alfalfa(Medicago truncatula)genome,and named MtOSCA1.1-MtOSCA4.1 according to their homology with AtOSCAs(Mt here denotes M.truncatula,and At denotes Arabidopsis thaliana).Chromosomal location analysis showed that the 13 MtOSCA genes were unevenly distributed on the 8 chromosomes.Furthermore,the MtOSCA family was phylogenetically divided into four subfamilies,and members from each subfamily were conserved in terms of intron-exon organization.Conserved functional domains and conserved motifs further indicated high conservatism of the MtOSCA family during evolution.Collinearity analysis suggested that MtOSCAs were closely related to GmOSCAs(Gm denotes Glycine max),but far from AtOSCAs.Gene expression pattern analysis revealed that MtOSCAs from different subfamilies exhibited tissue specificity.The expression of MtOSCA2.5/2.6/3.1 was found to be dramatically up-regulated by low temperature stress through transcriptome data and qRT-PCR analysis.In addition,the promoter sequences of MtOSCAs contained numbers of light-,hormone-,and stress-responsive cis-elements.In conclusion,results presented in this study lay a solid foundation for functional characterization of MtOSCA genes in regulating alfalfa tolerance to environmental stress.

关 键 词：蒺藜苜蓿 OSCA基因家族 全基因组鉴定 表达分析 

分 类 号：S542.9[农业科学—作物学]