纳米硒对滩羊生长性能、血液生化及代谢组的影响  

Effects of nano selenium on growth performance,blood biochemistry and metabolome of Tan sheep

作　　者：郭荣[1,2] 景海飞 杨仪 辛国省[2,3,4] 杨婷 GUO Rong;JING Hai-fei;YANG Yi;XIN Guo-sheng;YANG Ting(College of Animal Science and Technology,Ningxia University,Yinchuan 750021,China;Feed Engineering Technology Research Center of Ningxia University,Yinchuan 750021,China;Life and Sciences College,Ningxia University,Yinchuan 750021,China;Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Yinchuan 750021,China;Ningxia Haobiao Analysis&Test Institute,Yinchuan 750021,China)

机构地区：[1]宁夏大学动物科技学院,宁夏银川750021 [2]宁夏大学饲料工程技术研究中心,宁夏银川750021 [3]宁夏大学生命科学学院,宁夏银川750021 [4]宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021 [5]宁夏昊标检测服务研究院,宁夏银川750021

出　　处：《草业学报》2024年第9期199-213,共15页Acta Prataculturae Sinica

基　　金：优质富硒羊肉生产及高值转化关键技术研究与集成示范项目(2021BFH03006)资助。

摘　　要：本试验旨在探究不同添加水平的纳米硒对滩羊生长性能、血液生化、抗氧化及代谢组的影响。选取3月龄健康育肥公滩羊60只,按体重相近原则随机分为4组,每组15只,每组3个重复,每个重复5只羊:CON组(含硒0.02 mg·kg^(-1) DM,对照组)饲喂基础日粮,Ⅰ、Ⅱ和Ⅲ组(试验组)滩羊在饲喂基础日粮基础上分别添加0.1、0.3和0.5 mg·kg^(-1)纳米硒。预试期10 d,正试期90 d。结果表明:1)滩羊生长性能组间无显著差异(P>0.05)。2)Ⅰ组滩羊的经济效益(190.52元)高于其他组(187.85、174.92、131.01元)。3)试验组滩羊的血清丙氨酸氨基转移酶活性和尿素含量分别为13.75、13.25 U·L^(-1);8.26、8.11、7.52 mmol·L^(-1);显著低于CON组(23.00 U·L^(-1)、10.19 mmol·L^(-1);P<0.05)。4)Ⅲ组滩羊的血清碱性磷酸酶活性(494.75 U·L^(-1))显著高于其他组(262.75、228.00、250.50 U·L^(-1);P<0.05)。5)Ⅱ组血清球蛋白含量、总抗氧化能力和谷胱甘肽过氧化物酶活性(42.48 g·L^(-1)、9.91 U·m^(-1)、183.93 U·m^(-1))显著高于对照组(34.13 g·L^(-1)、4.93 U·m^(-1)、89.53 U·m^(-1);P<0.05)。6)通过血液代谢组学分析共筛选出21种差异显著代谢产物,主要富集的代谢通路有泛酸和辅酶A生物合成、碳水化合物消化和吸收、维生素消化和吸收等。综上所述,饲粮中添加纳米硒可改善滩羊的机体健康状况,提高抗氧化能力,这可能与代谢通路调控有关;本试验中,滩羊饲料纳米硒最适添加量为0.3 mg·kg^(-1)。This experiment was conducted to study the effects of different nano-selenium levels on growth rate,blood biochemistry and antioxidant function and metabolome of Tan sheep.Sixty 3-month-old healthy fattening Tan sheep with the similar body weight were randomly assigned to four groups with 15 sheep in each,and three replicates of five sheep per group.As a control(CON)sheep in one group were fed a basal diet(selenium content 0.02 mg·kg^(-1) DM,control group)while sheep in the other three‘test’groups(designatedⅠ,ⅡandⅢ)were fed the basal diet with an additional 0.1,0.3 and 0.5 mg·kg^(-1) of nano-selenium,respectively.A pre-trial adjustment period lasted for 10 days and the experimental period lasted for 90 days.It was found that:1)There were no significant group differences in growth rates of the Tan sheep(P>0.05).2)The economic return from groupⅠ(190.52 CNY)was higher than that in other groups(187.85,174.92 and 131.01 CNY for Con,groupⅡand groupⅢ,respectively).3)The contents of serum alanine aminotransferase(ALT)in test groupsⅡandⅢwere 13.75 and 13.25 U·L^(-1) compared with 23.00 U·L^(-1) in Con(P<0.05),and an intermediate value of 18.50 U·L^(-1) in GroupⅢ;contents of urea were 8.26,8.11,7.52 mmol·L^(-1),in groupsⅠ,ⅡandⅢ,respectively;significantly lower than the CON group(10.19 mmol·L^(-1))(P<0.05).4)The serum alkaline phosphatase(ALP)activity of Tan sheep in groupⅢ(494.75 U·L^(-1))was significantly higher than that in other groups(262.75,228.00,250.50 U·L^(-1) for groups CON,ⅠandⅡ,respectively)(P<0.05).5)GroupⅡserum globulin(GLOB),total antioxidant capacity(T-AOC)and glutathione peroxidase(GSH-Px)activity(42.48 g·L^(-1),9.91 U·m^(-1) and 183.93 U·m^(-1),respectively)were significantly higher than those of the CON group where corresponding values were 34.13 g·L^(-1),4.93 U·m^(-1) and 89.53 U·m^(-1)(P<0.05).6)A total of 21 metabolites with significant differences were screened through blood metabolomics analysis,with enrichment detected mainly in the metabolic pathwa

关 键 词：硒 宁夏滩羊 血清 抗氧化 代谢组 

分 类 号：S826.5[农业科学—畜牧学]