接种蚯蚓和腐解菌对秸秆腐解及土壤矿物结合有机碳形成的影响  

Effects of earthworms and straw-decomposing microbial inoculants on straw decomposition and soil mineral-associated organic carbon formation

作　　者：裴诗宇 杨家伟 吴彬[1] 赵叶新 叶成龙[1] 胡水金 PEI Shiyu;YANG Jiawei;WU Bin;ZHAO Yexin;YE Chenglong;HU Shuijin(College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China;Department of Entomology and Plant Pathology,North Carolina State University,Raleigh 27695,USA)

机构地区：[1]南京农业大学资源与环境科学学院,江苏南京210095 [2]北卡罗来纳州立大学昆虫和植物病理学系,美国北卡罗来纳州罗利27695

出　　处：《江苏农业学报》2024年第8期1424-1433,共10页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省自然科学基金项目(BK20200552)。

摘　　要：为了探究接种蚯蚓和腐解菌对秸秆分解及土壤矿物结合有机碳形成的影响,本研究以商品腐解菌和赤子爱胜蚓为接种物,分别设置仅添加秸秆(CK)、添加秸秆并接种腐解菌(SM)、添加秸秆并接种赤子爱胜蚓(SE)和添加秸秆并同时接种腐解菌和赤子爱胜蚓(SME)的试验处理,探讨腐解菌和蚯蚓的交互作用对土壤温室气体排放、细菌和真菌丰度、团聚体结构、金属离子含量、颗粒有机碳含量及矿物结合有机碳含量的影响及潜在机制。结果表明:(1)与CK相比,SE和SME处理显著增加了秸秆的腐解率;(2)与CK相比,SE和SME处理显著促进了土壤CO_(2)和N_(2)O的排放;SE和SM处理显著增加了土壤细菌的丰度,但是各处理间土壤真菌丰度无显著差异;(3)与CK相比,SE和SME处理显著增加了土壤中可提取钙镁含量,但是各处理间的非晶型铁铝含量和团聚体结构无显著差异;(4)与CK相比,SE和SME处理显著增加了土壤矿物结合有机碳和钙镁结合碳的含量,但是各处理间颗粒有机碳、铁铝结合碳的含量无显著差异;(5)线性回归分析结果表明,土壤钙镁结合碳含量与土壤可提取钙镁含量显著正相关,土壤矿物结合有机碳含量与细菌基因拷贝数呈正相关。综上所述,蚯蚓主导了秸秆的腐解和矿物结合有机碳的形成,蚯蚓和腐解菌的交互作用没有进一步促进秸秆腐解转化为土壤矿物结合有机碳。To explore the impact of earthworms and straw-decomposing microbial inoculants on straw decomposition and the formation of soil mineral-associated organic carbon,we conducted an incubation experiment with four treatments,i.e.,soil+straw(CK),soil+straw+straw-decomposing microbial inoculant(SM),soil+straw+earthworms(SE)and soil+straw+straw-decomposing microbial inoculant+earthworms(SME),to investigate interactive effects of straw-decomposing microbial inoculants and earthworms on soil greenhouse gas emission,bacterial and fungal abundance,soil aggregate structure,metal ion content,particulate organic carbon content and mineral-associated organic carbon content.The results indicated that compared with CK,SE and SME treatments significantly increased the straw decomposition rate and promoted the emission of CO_(2)and N_(2)O in soil.In addition,SE and SM treatments significantly increased bacterial abundance compared with CK,while there was no significant difference in soil fungal abundance among treatments.Compared with CK,SE and SME treatments significantly increased the contents of extractable calcium and magnesium in soil,and significantly facilitated the formation of mineral-associated organic carbon and Ca/Mg-associated carbon.But there were no significant differences in aggregate structure and the contents of non-crystalline Fe and Al,particulate organic carbon and Fe/Al-associated carbon among treatments.Linear correlation analysis showed that mineral-associated organic carbon formation and Ca/Mg-associated carbon were positively related with bacterial gene copy number and content of soil extractable Ca and Mg,respectively.In conclusion,earthworms dominated straw decomposition and mineral-associated organic carbon formation,and the interactions between earthworms and straw-decomposing microbial inoculants did not further promote the decomposition of straw into mineral-bound organic carbon.

关 键 词：土壤有机碳 土壤活性矿物 秸秆还田 腐解菌 蚯蚓 

分 类 号：Q148[生物学—生态学] S152[生物学—普通生物学]