基于转录组测序的乌桕叶色相关基因的挖掘  

Mining of genes related to Triadica sebifera leaf color based on transcriptome sequencing

作　　者：王晓晓 郑旭 赵文静 缪美华[1] 刘兴满[1] WANG Xiaoxiao;ZHENG Xu;ZHAO Wenjing;MIAO Meihua;LIU Xingman(Lianyungang Academy of Agricultural Sciences,Lianyungang 222000,China)

机构地区：[1]连云港市农业科学院,江苏连云港222000

出　　处：《江苏农业学报》2024年第8期1521-1532,共12页Jiangsu Journal of Agricultural Sciences

基　　金：中央财政林业科技推广示范基金项目[苏(2021)TG12];江苏省苏北科技专项(SZ-LYG202142);连云港市财政局专项基金项目(QNJJ2205)。

摘　　要：乌桕(Triadica sebifera)耐盐碱能力强,叶色随季节变化显著,尤以秋季观赏性最佳,是园林绿化中常用的彩叶植物。为揭示乌桕叶片呈色的分子机制,本研究对自主选育的3个乌桕品种连桕1号、云台红桕、云台金桕转色前后的叶片进行转录组测序,共获得123.78 GB测序数据,70815个高质量单一基因序列(Unigenes),其中48367个Unigenes得到了功能注释。将3个乌桕品种转色前后的转录组数据进行两两对比,各比较组之间鉴定出共有的差异表达基因(DEG)2674个。GO分析发现,DEG主要与细胞过程、细胞、结合等生物学功能相关。通过KEGG Pathway功能分析,筛选出53个与乌桕叶色相关的DEG,包括与花青素合成有关的基因14个,与卟啉和叶绿素代谢有关的基因22个,与类胡萝卜素生物合成有关的基因17个。其中花青素合成途径相关基因在乌桕转色后相对表达量上升,叶绿素、类胡萝卜素合成途径相关基因相对表达量下降,而叶绿素、类胡萝卜素降解途径相关基因相对表达量上升,该结果与叶片中色素含量的变化趋势一致。本研究结果可为探究乌桕叶片呈色机制提供理论依据。Triadica sebifera is a frequently-used color-leafed plant in landscaping because of its strong salt-tolerance and remarkable leaf color change with seasons,and it has the best ornamental properties in autumn.In order to reveal the molecular mechanism of leaf coloration of Triadica sebifera,the transcriptomes of leaves of three self-selected varieties of Triadica sebifera(Lianjiu No.1,Yuntaihongjiu and Yuntaijinjiu)were sequenced before and after leaf color changed.A total of 123.78 GB sequencing data were obtained,with 70815 high-quality Unigenes,of which 48367 were functionally annotated.A total of 2674 shared differentially expressed genes(DEGs)of different comparison groups were identified from the transcriptome data of three Triadica sebifera varieties before and after color transformation,through pairwise comparison.Go analysis revealed that DEGs were mainly related to biological functions such as Cell process,Cell and Binding.Through functional analysis of KEGG Pathway,53 DEGs related to leaf color were screened,including 14 genes related to anthocyanin synthesis,22 genes related to porphyrin and chlorophyll metabolism and 17 genes related to biosynthesis of carotenoid.The relative expression levels of genes related to anthocyanin biosynthesis pathway were increased and those related to chlorophyll and carotenoid biosynthesis pathways were decreased,the relative expression levels of genes related to chlorophyll and carotenoid degradation pathways were increased,which results were consistent with the change trends of pigment content in leaves.The results of this study can provide a theoretical basis for exploring the mechanism of leaf coloration of Triadica sebifera.

关 键 词：乌桕 叶色变化 转录组 基因挖掘 分子机制 呈色 

分 类 号：S687.9[农业科学—观赏园艺]