PNA捕获探针的合成及捕获肺癌EGFR突变体cfDNA的性能研究  

Synthesis of PNA Capture Probe and Study on Its Performance in Capturing Lung Cancer EGFR Mutant cfDNA

作　　者：徐小军 虞贤浩 孙思娴 Xu Xiaojun;Yu Xianhao;Sun Sixian(Zhejiang Pharmaceutical College,Ningbo 315100,China)

机构地区：[1]浙江药科职业大学,浙江宁波315100

出　　处：《广东化工》2024年第15期52-54,共3页Guangdong Chemical Industry

基　　金：浙江省自然科学基金基础公益研究计划项目(LGF21H180012);宁波市自然科学基金项目(2019A610190);校级重点科研课题(XZ2020001);浙江省大学生科技创新活动计划(新苗人才计划)项目(2022R460A003)。

摘　　要：肽核酸(peptidenucleicacid,PNA)寡聚物是一种合成的具有非天然聚酰胺骨架DNA类似物,因耐酸、耐酶和特异性杂交能力强而广泛应用于基因疾病的诊疗。然而关于肺癌的cfDNA基因检测目前仍然以传统的PCR扩增技术结合基因测序技术为主,检测时间长且费用较高,尤其是对于检测人员的专业技术要求较高。为解决以上问题,本课题应用固相合成技术制备PNA捕获探针,结合滚环扩增技术来研究PNA探针对肺癌EGFR突变体cfDNA的捕获性能,从而探索针对肿瘤cfDNA新的检测方法。经研究表明合成得到的PNA捕获探针能特异性识别并捕获pM级别EGFR突变体cfDNA,捕获性能满足检测方法灵敏度和精确度的要求,为肺癌的cfDNA基因检测新方法的建立提供关键技术,也为其他疾病的cfDNA基因检测提供技术支持。Peptide nucleic acid(PNA)oligomer is a synthetic DNA analog with a non-natural polyamide backbone.It is widely used in the diagnosis and treatment of genetic diseases due to its strong acid resistance,enzyme resistance and specific hybridization ability.However,cfDNA gene detection for lung cancer is still based on traditional PCR amplification technology combined with gene sequencing technology.The detection time is long and the cost is high,especially for the professional and technical requirements of the detection personnel.In order to solve the above problems,this topic solid-phase synthesis technology was used to prepare PNA capture probe,and rolling circle amplification technology was combined to study the capture performance of PNA probe for lung cancer EGFR mutant cfDNA,thereby exploring new detection methods for tumor cfDNA.Studies have shown that the synthesized PNA capture probe can specifically recognize and capture pM-level EGFR mutant cfDNA.The capture performance meets the sensitivity and accuracy requirements of the detection method,and provides key technology for the establishment of new cfDNA gene detection methods for lung cancer.Provide technical support for cfDNA genetic testing of other diseases.

关 键 词：PNA捕获探针 肺癌EGFR突变体cfDNA 捕获性能 

分 类 号：TQ[化学工程]