UPLC-MSMS法在西洋参、人参、红参中3种人参皂苷的含量研究与应用  

Research and Application for Contents Differentiation of Three Ginsenoside Compounds in Ginseng,Red Ginseng,American Ginseng and by UPLC-MS/MS

作　　者：郭海滨 张艳萍 黄婉锋 周宗洲 Guo Haibin;Zhang Yanping;Huang Wanfeng;Zhou Zongzhou(Foshan Institute for Drug and Food Control,Foshan 528051,China)

机构地区：[1]广东省佛山市食品药品检验检测中心,广东佛山528051

出　　处：《广东化工》2024年第16期184-186,137,共4页Guangdong Chemical Industry

基　　金：含人参中药制剂全链条掺伪风险监测(2023GD01)。

摘　　要：目的:建立一种可以同时测定西洋参、人参、红参中3种人参皂苷的UPLC-MSMS法。方法:采用Waters ACQUITY BEH C18色谱柱(2.1 mm×100 mm,1.7μm),0.3 mL/min的流速,以0.1%甲酸水溶液和四氢呋喃-乙腈溶液(5∶95)为流动相进行梯度洗脱,使用电喷雾离子源(ESI),多反应监测(MRM)的模式。结果:拟人参皂苷F11、人参皂苷Rf、Re分别在0.005μg/mL~0.20μg/mL、0.01μg/mL~0.40μg/mL、0.01μg/mL~0.40μg/mL的浓度范围内,呈良好的线性关系;3种人参皂苷的平均回收率分别为93.9%(RSD=1.9%)、91.9%(RSD=2.3%)和101.3%(RSD=4.1%),检出限分别为0.0005μg/mL、0.001μg/mL、0.003μg/mL,定量限分别为0.0017μg/mL、0.003μg/mL、0.010μg/mL。结论:该法具有准确,快速,灵敏,简便的特点,适用于西洋参、人参、红参3种人参皂苷化合物含量测定。Objective:To establish an UPLC-MSMS method to detect the contents of three ginsenoside compounds in Ginseng,Red Ginseng,American Ginseng.Methods:Waters ACQUITY BEH C18 column(2.1 mm×100 mm,1.7μm)was used as the chromatographic column,0.1%formic acid and tetrahydrofuran-acetonitrile(5∶95)was the mobile phasein gradient elution,and the flow rate was 0.3 mL/min.The Multiple reactions monitoring(MRM)mode was used as the detection mode,with electro-spray ionization(ESI)source.Results:The linear relationships of pseudoginsenoside F11,ginsenoside Rf,Re were good between 0.005μg/mL~0.20μg/mL,0.01μg/mL~0.40μg/mL,0.01μg/mL~0.40μg/mL;the average recoveries were 93.9%(RSD=1.9%),91.9%(RSD=2.3%)and 101.3%(RSD=4.1%),the limits of detections(LODS)were 0.0005μg/mL,0.001μg/mL,0.003μg/mL.The limits of quantitation(LOQS)were 0.0017μg/mL,0.003μg/mL,0.010μg/mL.Conculsion:The method is accurate,rapid,sensitive and convenient,can be used for quantitative determination of three ginsenoside compounds in Ginseng,Red Ginseng,American Ginseng.

关 键 词：UPLC-MS/MS 拟人参皂苷F11 人参皂苷Rf 人参皂苷RE 西洋参 人参 红参 

分 类 号：TQ[化学工程]