沙旋覆花石油醚部位洗脱组分的体外抗肿瘤活性研究  

Anti-Tumor Activity in vitro of Elution Components from Petroleum Ether Extract of Inula salsoloides(Turcz.)Ostenf.

作　　者：赵婷婷 高瑞娟[3] 郭君婷 叶斯木·塔拉甫别克 刘桂花 ZHAO Tingting;GAO Ruijuan;GUO Junting;TALPBEK Yesem;LIU Guihua(Xinjiang Institute of Materia Medica,Urumqi 830004,China;Xinjiang Key Laboratory of Uygur Medical Research,Urumqi 830004,China;Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences,Beijing 100050,China)

机构地区：[1]新疆维吾尔自治区药物研究所,新疆乌鲁木齐830004 [2]新疆维吾尔药重点实验室,新疆乌鲁木齐830004 [3]中国医学科学院医药生物技术研究所,北京100050

出　　处：《化学与生物工程》2024年第9期23-27,33,共6页Chemistry & Bioengineering

基　　金：新疆维吾尔自治区青年科技人才-乡村振兴项目(WJWY-XCZX202206);新疆维吾尔自治区公益性科研院所基本业务费专项资金项目(KY2022144);新疆维吾尔自治区重点实验室项目(2023D04035)。

摘　　要：采用CCK-8法从沙旋覆花中筛选出具有抗肿瘤活性的石油醚部位洗脱组分,研究了其对人食管癌细胞Eca109增殖的抑制作用,并通过4′,6-二脒基-2-苯基吲哚(DAPI)染色法探究了其对Eca109细胞抑制作用的方式。结果表明,沙旋覆花石油醚部位洗脱组分(除23^(#)外)对Eca109细胞具有不同程度的抑制作用,其中71^(#)组分的抑制作用最强,对Eca109细胞的IC_(50)值为8.19μg·mL^(-1);沙旋覆花石油醚部位洗脱组分可通过诱导细胞裂亡和凋亡发挥抗食管癌作用,为后续开展旋覆花属植物抗食管癌活性成分筛选与药效评价奠定了基础。We screened out elution components from the petroleum ether extract of Inula salsoloides(Turcz.)Ostenf.with anti-tumor activity by CCK-8 method,studied its inhibitory effect on the proliferation of human esophageal cancer cells Eca109,and investigated the inhibitory mode by DAPI staining.The results reveal that the elution components(except 23^(#))show different inhibitory effects on Eca109 cells,among which component 71^(#)has the strongest inhibitory effect,and the IC 50 value to Eca109 cells is 8.19μg·mL^(-1).The elution components from petroleum ether extract of Inula salsoloides(Turcz.)Ostenf.can play an anti-esophageal cancer role by inducing cell fission and apoptosis.The study lays a foundation for screening and pharmacodynamic evaluation of anti-esophageal cancer active components of Inula L..

关 键 词：沙旋覆花 石油醚部位 体外抗肿瘤活性 CCK-8法 

分 类 号：R285.5[医药卫生—中药学]