多种金属有机框架材料的类过氧化物酶活性研究  

Peroxidase-Like Activity of a Variety of Metal-Organic Framework Materials

作　　者：邵路瑶 王志刚 孙璇 SHAO Luyao;WANG Zhigang;SUN Xuan(School of Basic Medical Sciences,Hubei University of Chinese Medicine,Wuhan 430056,China)

机构地区：[1]湖北中医药大学基础医学院,湖北武汉430056

出　　处：《化学与生物工程》2024年第9期34-37,47,共5页Chemistry & Bioengineering

基　　金：湖北省自然科学基金青年项目(2024AFB375);湖北中医药大学博士启动经费项目(2023ZXB015);湖北中医药大学创新创业训练计划项目(S202310507005)。

摘　　要：采用溶剂热法合成了ZIF-8、UIO-66、MIL-101(Fe)、MIL-101(Cr)、Cu-TCPP(Fe)等5种金属有机框架(MOF)材料,通过观察MOF材料与3,3′,5,5′-四甲基联苯胺(TMB)反应的颜色变化及测定反应液的OD_(450/630)值评价其类过氧化物酶活性。结果表明,ZIF-8、MIL-101(Fe)、MIL-101(Cr)几乎没有类过氧化物酶活性,UIO-66的类过氧化物酶活性微弱,而二维片状的Cu-TCPP(Fe)显示出超高的类过氧化物酶活性;Cu-TCPP(Fe)的类过氧化物酶活性来源于TCPP(Fe),其类血红素结构能加速TMB反应的颜色变化。MOF材料作为人工模拟酶在纳米生物学领域显示出巨大的应用潜力。We synthesized five metal-organic framework(MOF)materials,including ZIF-8,UIO-66,MIL-101(Fe),MIL-101(Cr),and Cu-TCPP(Fe),by a solvent-thermal method.Moreover,we evaluated the peroxidase-like activity by the observation of the color change of the reaction between MOF materials and 3,3′,5,5′-tetramethylbenzidine(TMB)and by the measurement of the OD_(450/630)value of the reaction solution.The results show that ZIF-8,MIL-101(Fe),and MIL-101(Cr)have hardly any peroxidase-like activity,UIO-66 has weak peroxidase-like activity,while Cu-TCPP(Fe)shows high peroxidase-like activity.In addition,the peroxidase-like activity of Cu-TCPP(Fe)is mainly derived from TCPP(Fe),and the heme-like structure of TCPP(Fe)can accelerate the color change of TMB reaction.MOF materials have shown great potential as artifically simulated enzymes in the field of nanobiology.

关 键 词：金属有机框架 Cu-TCPP(Fe) 类过氧化物酶 3 3′ 5 5′-四甲基联苯胺 显色反应 

分 类 号：TQ426.97[化学工程]