基于线粒体COI基因鉴定技术的盐城市长角血蜱遗传多样性分析  

Genetic diversity analysis of Haemaphysalis longgicornis in Yancheng based on mitochondrial gene COI identification technology

作　　者：陈国清[1] 崔倩[1] 张红军[1] 李峰[1] 李春香[2] 燕清丽[3] 王富华 徐兵 安然 杨鹏飞[3] CHEN Guoqing;CUI Qian;ZHANG Hongjun;Li Feng;LI Chunxiang;YAN Qingli;WANG Fuhua;XU Bing;AN Ran;YANG Pengfei(Yancheng Center for Disease Control and Prevention,Yancheng,Jiangsu 224000,China;不详)

机构地区：[1]盐城市疾病预防控制中心,江苏盐城224000 [2]盐城市妇幼保健院 [3]淮安市疾病预防控制中心

出　　处：《中国国境卫生检疫杂志》2024年第4期358-362,共5页Chinese Journal of Frontier Health and Quarantine

基　　金：江苏省卫健委预防医学科研课题(Ym2023024);江苏省血地寄防科研课题(x202340);新发突发重大传染病病原微生物重点实验室(江苏省疾病预防控制中心)开放基金课题(PM202402);盐城市医学科研课题(YK2023085)。

摘　　要：目的了解盐城市长角血蜱的遗传多样性和不同地理种群间系统发育关系,为该地区长角血蜱及其蜱媒病的防控提供理论基础。方法2022年10月—2023年4月,在盐城市亭湖(枸杞灌木丛、盐地碱蓬区、海堤草丛、白茅、居民区)、东台(河堤草丛)两地共6种生境采集蜱虫,形态学鉴定后用普通PCR扩增线粒体细胞色素C氧化酶I亚基(COI)基因,进行分子鉴定。结果共采集38只蜱虫,包括25只成蜱和13只若蜱,经形态学与分子生物学鉴定均为长角血蜱,分布于两个进化分支,主要进化分支位于Compound分支中。不同地理种群分属于5个共享单倍型,25个为独享单倍型,其中盐城市共涉及3个共享单倍型,10个单倍型,H5、H10处于辐射中心位置,可能较为原始。结论本次采集的长角血蜱在不同地理种群无明显分化,在盐城种群中可能处于扩张突变积累中。Objective To determine the genetic diversity of Haemaphysalis longgicornis and phylogenetic relationships among different geographic populations,in order to provide a basis for the prevention and control of H.longgicornis and tick-borne diseases.Methods Morphological identification was conducted on ticks collected from Sheyang and Dongtai of Yancheng City from October 2022 to April 2023,including 6 kinds of habitats such as goji berry shrubs,saline alkali marsh areas,seawall grass,white thatch,residential areas in Tinghu,and riverbank grass in Dongtai.The molecular identification was performed through conventional PCR for amplifying the mitochondrial cytochrome C oxidase I subunit(COI)gene.Results The 38 collected ticks(25 adult ticks and 13 nymphs)in this study were identified as H.longgicornis by morphology and molecular biology methods.The H.longicornis collected in Yancheng were distributed in two evolutionary branches,with the main evolutionary branch located in the"Compound"branch.Different geographical populations belonged to 5 shared haplotypes,with 25 being exclusive haplotypes.Among them,Yancheng was involved in a total of 3 shared haplotypes and 10 being exclusive haplotypes.In the network of haplotypes,H5 and H10 were found to be the primary haplotypes,they formed two radical centers,the representative sequence haplotypes in Yancheng were rich and colorful.Conclusion There was no significant differentiation among different geographical populations in this study,and H.longicornis population in Yancheng might be undergoing the accumulation of expansion and mutation.

关 键 词：长角血蜱 系统发育 种群遗传多样性 种群遗传结构 

分 类 号：R183.5[医药卫生—流行病学] R384.4[医药卫生—公共卫生与预防医学]