高效液相色谱波长切换法同时测定紫斑牡丹籽壳中6种成分含量  

Simultaneous determination of 6 components in the seed coat of Paeonia rockii by HPLC wavelength-switching method

作　　者：李洁 石晓峰 董学凤[2] 张家旭 叶倩女 范彬 王新娣 LI Jie;SHI Xiaofeng;DONG Xuefeng;ZHANG Jiaxu;YE Qiannyu;FAN Bin;WANG Xindi(Lanzhou Food and Drug Inspection and Testing Institute of Chinese Medicine Laboratory,Lanzhou,Gansu,730050,China;College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou,Gansu,730101,China;Gansu Academy of Medical Sciences,Lanzhou,Gansu,730050,China)

机构地区：[1]兰州市食品药品检验检测研究院中药检验室,甘肃兰州730050 [2]甘肃中医药大学药学院,甘肃兰州730101 [3]甘肃省医学科学研究院,甘肃兰州730050

出　　处：《甘肃中医药大学学报》2024年第4期1-7,共7页Journal of Gansu University of Chinese Medicine

基　　金：甘肃省药品监督管理局药品科研项目(2022GSMPA0070)。

摘　　要：目的建立高效液相色谱(HPLC)波长切换法同时测定紫斑牡丹籽壳中6种成分含量的方法。方法采用HPLC波长切换法同时测定紫斑牡丹籽壳中没食子酸、氧化芍药苷、芍药内酯苷、芍药苷、白藜芦醇、木犀草素6种成分的含量。色谱柱为CAPCELLPAK C_(18)(250.0 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液,梯度洗脱,流速1.0 mL/min,检测器为二极管阵列检测器(DAD),检测波长分别为273,260,230,228,305,350 nm,柱温30℃,进样量10μL。结果没食子酸、氧化芍药苷、芍药内酯苷、芍药苷、白藜芦醇、木犀草素的回归方程分别为:Y=30209X+193.54(r=0.9992),Y=16415X-118.55(r=0.9993),Y=8735.8X-5072.6(r=0.9992),Y=13658X-39219(r=0.9999),Y=82092X+25114(r=0.9990),Y=43038X+11152(r=0.9992),分别在0.2594~5.1886,0.7589~15.1782,1.2475~24.9500,11.7005~234.0093,3.3526~67.0513,2.7802~55.6032μg范围内线性关系良好;精密度、重复性、稳定性试验的RSD分别为0.7%~1.4%(n=6),0.7%~1.9%(n=6),0.5%~1.0%(n=8);平均加样回收率分别为99.87%,100.41%,99.60%,100.57%,100.29%,99.49%,RSD分别为2.0%,1.9%,1.8%,1.6%,1.8%,1.8%。结论该法具有提取简便、提取率高、重复性好、准确度高等特点,可作为紫斑牡丹籽壳中6种成分的定量分析方法。Objective To establish a high-performance liquid chromatography(HPLC)wavelength-switching approach for the concurrent determination of 6 components in the seed coat of Paeonia rockii.Methods The contents of gallic acid,oxypaeoniflorin,albiflorin,paeoniflorin,resveratrol and luteolin in the seed coat of Paeonia rockii were determined via the HPLC wavelength-switching method.The determination was conducted on a CAPCELLPAK C_(18) column(250.0 mm×4.6 mm,5μm)with the mobile phase composed of acetonitrile-0.1%phosphoric acid solution at a gradient elution rate of 1.0 mL/min.The detector used was a diode array detector(DAD),and the detection wavelengths were set at 273,260,230,228,305,350 nm,respectively.The column temperature was set at 30℃,and the sample volume was 10μL.Results The regression equations of gallic acid,oxypaeoniflorin,albiflorin,paeoniflorin,resveratrol and luteolin were as follows:Y=30209X+193.54(r=0.9992),Y=16415X-118.55(r=0.9993),Y=8735.8X5072.6(r=0.9992),Y=13658X-39219(r=0.9999),Y=82092X+25114(r=0.9990),Y=43038X+11152(r=0.9992),within the ranges of 0.2594 to 5.1886,0.7589 to 15.1782,1.2475 to 24.9500,11.7005 to 234.0093,respectively.Good linear relationships were observed within the ranges of 3.3526 to 67.0513 and 2.7802 to 55.6032μg.The RSDs of the precision,repeatability and stability tests were 0.7%~1.4%(n=6),0.7%~1.9%(n=6)and 0.5%~1.0%(n=8),respectively.The average recoveries were 99.87%,100.41%,99.60%,100.57%,100.29%and 99.49%,respectively,and the RSDs were 2.0%,1.9%,1.8%,1.6%,1.8%and 1.8%.Conclusion This method features simple extraction,high extraction rate,good repeatability and high accuracy,and can be utilized for the quantitative analysis of the six components in the seed coat of Paeonia rockii.

关 键 词：紫斑牡丹籽壳 没食子酸 氧化芍药苷 芍药内酯苷 芍药苷 白藜芦醇 木犀草素 高效液相色谱 含量测定 

分 类 号：R284.2[医药卫生—中药学]