防风外观性状与指标成分相关性研究  

Study on the correlation between appearance traits and index components of Saposhnikovia Radix

作　　者：杨丽 潘礼业 索彩仙 曾荟 童培珍 胡绮萍 李国卫 YANG Li;PAN Liye;SUO Caixian;ZENG Hui;TONG Peizhen;HU Qiping;LI Guowei(Guangdong Yifang Pharmaceutical Co.Ltd,Foshan,Guangdong,528244,China;Key Laboratory of Guangdong Provincial TCM Formula Enterprise,Foshan,Guangdong,528244,China)

机构地区：[1]广东一方制药有限公司,广东佛山528244 [2]广东省中药配方颗粒企业重点实验室,广东佛山528244

出　　处：《甘肃中医药大学学报》2024年第4期31-36,共6页Journal of Gansu University of Chinese Medicine

基　　金：佛山市核心技术攻关项目——产业关键技术项目(1920001000378);国家工业和信息化部消费品工业司2019年产业技术基础公共服务平台项目(2019-00902-1-2)。

摘　　要：目的探讨防风外观性状与指标成分之间的相关性,为防风药材商品规格等级的划分提供科学依据。方法采集内蒙古、河北及黑龙江产区的防风样品,观察并量化其直径、质地等性状指标;采用高效液相色谱(HPLC)法测定防风中升麻素苷及5-O-甲基维斯阿米醇苷的含量;采用SPSS 22.0统计软件对防风外观性状及指标成分含量进行相关性分析,进一步比较同一批次不同直径防风药材的质量,验证其直径与指标成分含量之间的相关性。结果不同产地、不同生长方式的防风药材在外观性状及指标成分含量方面均存在较大差异;指标成分含量与药材质地呈显著正相关(P<0.01),而与直径呈负相关,即质地松、直径小的防风药材中升麻素苷及5-O-甲基维斯阿米醇苷含量较高;根头环纹与质地呈显著正相关(P<0.05);药材质量与“扫帚头”没有明显相关性。结论防风外观性状与指标成分含量具有相关性,可根据直径大小、质地等性状直接预测药材的质量,为防风药材的质量等级评价奠定基础。Objective To explore the correlation between the appearance traits and index components of Saposhnikovia Radix and to provide scientific basis for the classification of commercial specifications of Saposhnikovia Radix.Methods The samples were collected from Inner Mongolia,Hebei and Heilongjiang,and the appearance characteristic indexes were observed and quantified,such as the diameter and texture.The HPLC method was used to determine the content of prim-O-glucosylcimifugin and 5-O-methylvisammioside.SPSS 22.0 statistical software was used to conduct correlation analysis on the appearance traits and the content of index components,and further compared the quality of the same batch with different diameters to verify the correlation between their diameters and the content of index components.Results There were great differences in the appearance traits and the content of the index components among the Saposhnikovia Radix from different regions and of different growing modes.The content of index components was significantly positively correlated with the texture(P<0.01),but negatively correlated with the diameter,i.e.,the content of prim-O-glucosylcimifugin and 5-O-methylvisammioside in Saposhnikovia Radix with loose-texture and small diameter was higher;the correlation between root ring pattern and texture is significantly positive(P<0.05).There was no obvious correlation between the quality of medicinal materials and its"broomhead".Conclusion There is a certain correlation between the appearance traits and the content of index components of Saposhnikovia Radix,and its quality can be predicted directly according to its diameter,texture and other characters,laying a foundation for the quality grade evaluation of Saposhnikovia Radix.

关 键 词：防风 外观性状 升麻素苷 5-O-甲基维斯阿米醇苷 高效液相色谱 含量测定 

分 类 号：R284.2[医药卫生—中药学]