基于OCT的活体斑马鱼生长发育特征研究  

Growth and Developmental Characteristics of Zebrafish Based on Optical Coherence Tomography

作　　者：杜小玉 李介福 赵潮 舒宇康 赵航 史晓凤[1] 马君[1] Du Xiaoyu;Li Jiefu;Zhao Chao;Shu Yukang;Zhao Hang;Shi Xiaofeng;Ma Jun(Qingdao Key Laboratory of Optical Optoelectronics,College of Physics and Optoelectronic Engineering,Faculty of Information Science and Engineering,Ocean University of China,Qingdao 266100,Shandong,China)

机构地区：[1]中国海洋大学信息科学与工程学部物理与光电工程学院青岛市光学光电子重点实验室,山东青岛266100

出　　处：《激光与光电子学进展》2024年第14期227-236,共10页Laser & Optoelectronics Progress

基　　金：国家自然科学基金青年科学基金(42206191);山东省重点研发计划项目(2019GHY112027)。

摘　　要：作为生物医学领域的理想动物模型,斑马鱼的生长发育过程具有较高研究价值。利用光学相干层析(OCT)技术对斑马鱼的发育进行三维活体连续成像监测,建立斑马鱼生长发育的统一模型,同时对其眼、脑、心脏等主要器官形态进行量化分析。结果表明,斑马鱼在胚胎期主要完成了从卵膜内到膜外发育的转变,在仔鱼期外形和内部结构变化最为明显,在幼鱼期完成了基本器官的发育,在成鱼期性腺发育成熟。其中,斑马鱼的脑部各结构分区直至成鱼期才发育完善;眼睛在胚胎期主要为功能性发育,之后为形态生长;心室面积在发育阶段增大约60倍。该研究实现了对斑马鱼完整发育周期的活体成像和内部器官生长状况的表征,为以斑马鱼为模型的生长发育研究提供参考。Growth and development of zebrafish are valuable areas of research as these fish serve as an excellent animal model in biomedicine.This study used optical coherence tomography(OCT)to continuously monitor the threedimensional imaging of zebrafish development,creating a comprehensive model for their growth and development.Furthermore,the study evaluated the morphology of key organs,such as the eyes,brain,and heart,through quantitative analysis.The findings revealed that zebrafish undergo transition from inner to outer ovary development primarily during the embryonic phase,with the most significant changes in shape and internal composition occurring during the larval phase.Basic organ development is completed during the juvenile stage,whereas the gonads reach maturity during the adult stage.Although the brain does not reach full maturity until adulthood,eye development is primarily functional during the embryonic stage.This growth pattern is significantly different from that observed in other species.Moreover,during the developmental stage of zebrafish,there is an approximately 60-fold increase in the ventricular area.This study successfully captured in vivo images of the entire zebrafish developmental process and examined the growth status of its internal organs.These results provide crucial information for future studies on growth and development using zebrafish as a model.

关 键 词：生物光学 光学相干层析 斑马鱼 活体成像 生长发育 

分 类 号：Q954.3[生物学—动物学]