Phase-modulated multi-foci microscopy for rapid 3D imaging  

作　　者：WEIQI WANG LI GONG ZHIWEI HUANG 

机构地区：[1]Optical Bioimaging Laboratory,Department of Biomedical Engineering,College of Design and Engineering,National University of Singapore,Singapore 117576,Singapore

出　　处：《Photonics Research》2024年第7期1548-1555,共8页光子学研究（英文版）

基　　金：National Medical Research Council (NMRC),Singapore (A-0009502-01-00, A-8001143-00-00);Merlion Fund (R397-000-356-133);Academic Research Fund(AcRF) from the Ministry of Education (MOE),Singapore(Tier 1 (R397-000-334-114),Tier 1 (R397-000-371-114,R397-000-378-114),Tier 2 (A-8000117-01-00))。

摘　　要：3D imaging technology is pivotal in monitoring the functional dynamics and morphological alterations in living cells and tissues.However,conventional volumetric imaging associated with mechanical z-scanning encounters challenges in limited 3D imaging speed with inertial artifact.Here,we present a unique phase-modulated multifoci microscopy (PM^(3)) technique to achieve snapshot 3D imaging with the advantages of extended imaging depths and adjustable imaging intervals between each focus in a rapid fashion.To accomplish the tasks,we utilize a spatial light modulator (SLM) to encode the phases of the scattered or fluorescence light emanating from a volumetric sample and then project the multiple-depth images of the sample onto a single charge-coupled device camera for rapid 3D imaging.We demonstrate that the PM^(3)technique provides~55-fold improvement in imaging depth in polystyrene beads phantom compared to the depth of field of the objective lens used.PM^(3)also enables the real-time monitoring of Brownian motion of fluorescent beads in water at a 15 Hz volume rate.By precisely manipulating the phase of scattered light on the SLM,PM^(3)can pinpoint the specific depth information in living zebrafish and rapidly observe the 3D dynamic processes of blood flow in the zebrafish trunk.This work shows that the PM^(3)technique developed is robust and versatile for fast 3D dynamic imaging in biological and biomedical systems.

关 键 词：alterations SCATTERED BEADS 

分 类 号：R318[医药卫生—生物医学工程] TP391.41[医药卫生—基础医学]