电针治疗对帕金森病模型小鼠ERK/CREB/BDNF通路的调控作用  

Regulation of ERK/CREB/BDNF Pathway in Parkinson′s Disease Model Mice by Electroacupuncture Treatment

作　　者：荣臻 李亚楠 汪瑶 张小蕾 胡梦妮 马骏[1] RONG Zhen;LI Yannan;WANG Yao;ZHANG Xiaolei;HU Mengni;MA Jun(Hubei University of Chinese Medicine,Wuhan 430000,China)

机构地区：[1]湖北中医药大学,湖北武汉430000

出　　处：《中医药信息》2024年第9期7-12,共6页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473788);国家重点研发计划项目(2020YFC0845800)。

摘　　要：目的:观察电针“风府”“太冲”穴对帕金森病(PD)小鼠酪氨酸羟化酶(TH)以及ERK/CREB/BDNF通路的影响,探讨电针治疗PD的可能机制。方法:40只C57BL/6小鼠随机分为正常组、模型组、电针组和左旋多巴组,每组各10只。采用MPTP连续腹腔注射7 d的方法复制PD模型。MPTP腹腔注射1 h后,电针组电针“风府”“太冲”穴,30 min/次,1次/d,连续治疗12 d;正常组和模型组给予相同时间的抓取和固定;左旋多巴组灌胃25 mg/mL左旋多巴。观察各组小鼠行为学变化;免疫组化法检测黑质中酪氨酸羟化酶(TH)阳性表达;Western blot检测黑质中目的蛋白表达,荧光定量PCR检测目的因子mRNA相对表达量。结果:造模后,与正常组比较,模型组出现行为学异常,行为学评分升高(P<0.01),小鼠黑质TH阳性表达下降(P<0.05,P<0.01),小鼠ERK1/2、CREB、BDNF mRNA相对表达量下降(P<0.01,P<0.05),ERK1/2、CREB、BDNF蛋白表达下降(P<0.01)。治疗后,与模型组比较,电针组和左旋多巴组小鼠行为学均有所改善,行为学评分降低(P<0.05,P<0.01),黑质TH阳性表达上升(P<0.05),ERK1/2、CREB、BDNF mRNA相对表达量上调(P<0.01,P<0.05),ERK1/2、CREB、BDNF蛋白表达上调(P<0.01,P<0.05)。结论:电针“风府”“太冲”穴,可以提高PD小鼠TH表达,同时调控中脑黑质ERK/CREB/BDNF通路,改善PD小鼠的运动功能障碍。Objective:To observe the effects of electroacupuncture at Fengfu(GV16)and Taichong(LR3)acupoints on tyrosine hydroxylase(TH)expression and the ERK/CREB/BDNF pathway in mice with Parkinson′s disease(PD),and explore the potential mechanisms of electroacupuncture treatment for PD.Methods:Forty C57BL/6 mice were randomly divided into normal,model,electroacupuncture,and levodopa groups(n=10/group).The PD model was induced by intraperitoneal injection of MPTP for 7 days.In the electroacupuncture group,GV16 and LR3 were stimulated 1 hour after MPTP injection,once daily for 12 consecutive days.The normal and model groups received handling and fixation for the same duration.The levodopa group received oral levodopa at 25 mg/mL.Behavioral changes were observed in each group.Immunohistochemistry was used to detect TH-positive expression in the substantia nigra.Western blot and fluorescence quantitative PCR were employed to measure the protein and mRNA expression levels of target factors in the substantia nigra,respectively.Results:Following modeling,compared with the normal group,the model group exhibited behavioral abnormalities with increased behavioral scores(P<0.01),decreased THpositive expression in the substantia nigra(P<0.01),and downregulated mRNA(P<0.01,P<0.05)and protein expression(P<0.01)of ERK1/2,CREB,and BDNF in the substantia nigra.After treatment,compared with the model group,both the electroacupuncture and levodopa groups showed improved behavioral scores(P<0.05,P<0.01),increased TH-positive expression in the substantia nigra(P<0.05),and upregulated mRNA(P<0.01,P<0.05)and protein expression(P<0.01,P<0.05)of ERK1/2,CREB,and BDNF in the substantia nigra.Conclusion:Electroacupuncture at GV16 and LR3 acupoints can enhance TH expression and modulate the ERK/CREB/BDNF pathway in the substantia nigra of PD mice,thereby improving motor dysfunction in PD mice.

关 键 词：帕金森病 电针 ERK BDNF CREB 

分 类 号：R245[医药卫生—针灸推拿学]