我国部分地区A2dB1型传染性法氏囊病病毒新型变异株的鉴定及序列分析  

Identification and Sequence Analysis of A2dB1 Genotype Novel Variant Infectious Bursal Disease Virus in Some Regions of China

作　　者：张文英 于航博 姜楠 王国栋 牛鑫鑫 黄萌萌 张玉龙 韩金泽 许萌萌 刘长军[1] 王素艳 李凯[1] 高立[1] 崔红玉[1] 张艳萍[1] 陈运通 高玉龙[1,2] 祁小乐 ZHANG Wenying;YU Hangbo;JIANG Nan;WANG Guodong;NIU Xinxin;HUANG Mengmeng;ZHANG Yulong;HAN Jinze;XU Mengmeng;LIU Changjun;WANG Suyan;LI Kai;GAO Li;CUI Hongyu;ZHANG Yanping;CHEN Yuntong;GAO Yulong;QI Xiaole(Avian Immunosuppressive Diseases Division,State Key Laboratory for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,Heilongjiang 150001;World Organization for Animal Health(WOAH)Reference Laboratory for Infectious Bursal Disease,Harbin,Heilongjiang 150001)

机构地区：[1]中国农业科学院哈尔滨兽医研究所,动物疫病防控全国重点实验室,禽免疫抑制病创新团队,黑龙江哈尔滨150001 [2]世界动物卫生组织传染性法氏囊病参考实验室,黑龙江哈尔滨150001

出　　处：《中国家禽》2024年第9期194-200,共7页China Poultry

基　　金：黑龙江省自然科学基金项目(ZD2020C006);“十四五”黑龙江省重点研发计划项目(GA21B004);国家自然科学基金项目(32072852)。

摘　　要：为持续了解传染性法氏囊病病毒新型变异株(Novel variant infectious bursal disease virus,nVarIBDV)在我国流行情况,研究收集2021—2022年6个主要养禽地区疑似传染性法氏囊病(Infectious bursal disease,IBD)鸡病料,采用RT-PCR进行检测,并对11株IBDV代表毒株的基因组双节段代表区段VP2-HVR和VP1-B-marker进行基因测序及序列分析。结果显示:所检测病料的IBDV阳性率为20.65%(19/92),其中11株IBDV代表毒株均为A2dB1型nVarIBDV。研究表明,nVarIBDV在我国主要养禽地区仍持续流行,而且出现了值得注意的新的突变位点,有必要进行持续的IBDV流行病学监测。To continue understanding the prevalence of novel variant infectious bursal disease virus(nVarIBDV)in China,the suspected infectious bursal disease(IBD)samples were collected from six major poultry areas during 2021 to 2022.These samples were used for RT-PCR detection,and the representative fragments of VP2-HVR and VP1-B-marker of 11 representative IBDV strains were sequenced and analyzed.The results showed that the IBDV positive rate in the tested samples was 20.65%(19/92),and all the 11 representative strains belonged to A2dB1 genotype nVarIBDV.The results suggested that nVarIBDV was still prevalent in major poultry breeding areas in China,and notable new mutation sites had emerged,and continuous IBDV epidemio-logical surveillance was necessary.

关 键 词：传染性法氏囊病病毒 新型变异株 A2dB1型 流行病学 

分 类 号：S855.3[农业科学—临床兽医学]