电针对慢性前列腺炎大鼠TLR4/NF-κB/NLRP3通路蛋白表达的影响  

Impacts of Electroacupuncture on Protein Expressions of TLR4/NF-κB/NLRP3 Pathway in Rats with Chronic Prostatitis

作　　者：冯鑫鑫[1] 刘成勇[2] 鲍挺峰 张奕 陈雷[1] 秦珊[2] FENG Xinxin;LIU Chengyong;BAO Tingfeng;ZHANG Yi;CHEN Lei;QIN Shan(Ningbo Municipal Hospital of Traditional Chinese Medicine,Affiliated Hospital of Zhejiang Chinese Medical University,Ningbo 315000,Zhejiang,China;Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu,China)

机构地区：[1]浙江中医药大学附属宁波市中医院,浙江宁波315000 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《中华中医药学刊》2024年第9期162-166,I0031,I0032,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82205252);浙江省陈雷名老中医专家传承工作室建设项目(GZS2020038);宁波市自然科学基金项目(202003N4212);第四轮宁波市医学重点学科建设计划项目(2022-ZF01)。

摘　　要：目的观察电针对慢性前列腺炎大鼠前列腺Toll样受体4/核转录因子κB/NOD样受体蛋白3(TLR4/NF-κB/NLRP3)通路的影响,探讨电针治疗慢性前列腺炎的可能机制。方法将40只SPF级健康雄性SD大鼠随机分为空白组、模型组、电针组、抑制剂组、激动剂组,各8只。除空白组外,其余各组采用前列腺蛋白提取及免疫注射法制备大鼠慢性前列腺模型,于造模后1 d对电针组、抑制剂组及激动剂组大鼠行电针干预,穴取“白环俞”“会阳”,连续波,频率2 Hz,每日1次,每次20 min,7 d为1个疗程,每疗程后休息1 d,共治疗2个疗程。基于开野实验和糖水消耗实验开展行为学测试;取大鼠前列腺组织,计算前列腺湿重及前列腺指数;HE染色观察大鼠前列腺组织形态学变化;TUNEL法观察前列腺细胞凋亡;Western blot法检测前列腺组织中TLR4、NF-κB、NLRP3蛋白表达。结果与空白组比较,模型组大鼠水平运动评分、垂直运动评分和糖水消耗量均显著下降(P<0.05);与模型组比较,电针组大鼠水平运动评分、垂直运动评分和糖水消耗量显著增加(P<0.05);与电针组比较,抑制剂组大鼠水平运动评分、垂直运动评分和糖水消耗量显著增加,而激动剂组大鼠水平运动评分、垂直运动评分和糖水消耗量显著下降(P<0.05)。与空白组比较,模型组大鼠前列腺湿重和前列腺指数显著升高(P<0.05),前列腺细胞凋亡升高(P<0.05);与模型组比较,电针组大鼠前列腺湿重和前列腺指数显著下降(P<0.05),前列腺细胞凋亡显著下降(P<0.05);与电针组比较,抑制剂组大鼠前列腺湿重和前列腺指数显著下降(P<0.05),而激动剂组大鼠前列腺湿重和前列腺指数显著升高(P<0.05),且抑制剂组大鼠前列腺细胞凋亡进一步下降(P<0.05),而激动剂组大鼠前列腺细胞凋亡升高(P<0.05)。与空白组比较,模型组大鼠前列腺组织中TLR4、p-NF-κB/NF-κB、NLRP3蛋白表达显著增加(P<0.05),与模型�Objective To observe the effect of electroacupuncture on the Toll-like receptor 4/nuclear transcription factorκB/NOD-like receptor protein 3(TLR4/NF-κB/NLRP3)pathway in the prostate of rats with chronic prostatitis,and to explore the possible mechanisms of the electroacupuncture treatment of chronic prostatitis(CP).Methods Forty healthy male SD rats with SPF grade were randomly divided into the blank group,model group,electroacupuncture group,inhibitor group and agonist group with 8 rats in each.Except the blank group,the rest of the groups were given prostatic protein extraction and immunization injec-tion to prepare rat CP models.One day after model establishment,the rats in the electroacupuncture group,inhibitor group and agonist group were intervened by electroacupuncture at“Baihuanshu(BL30)”and“Huiyang(BL35)”.Continuous waves with a frequency of 2 Hz were given once a day,20 min for each time,7 days as a course of treatment.There was a total of 2 courses of treatment with 1 day break in the middle.Behavioral tests were carried out based on the open-field test and sugar water con-sumption test.The rat prostate tissue was collected,and the wet weight and prostate index of the prostate were calculated.HE staining was used to observe the morphological changes of the rat prostate tissue.TUNEL method was used to observe the apopto-sis of prostate cells.Western blot method was used to detect the expression sof TLR4,NF-κB and NLRP3 proteins in prostate tissue.Results Compared with those in the blank group,the horizontal movement score,vertical movement score and sugar water consumption of rats in the model group were significantly decreased(P<0.05).Compared with those in the model group,the horizontal movement score,vertical movement score and sugar water consumption of the rats in the electroacupuncture group were significantly increased(P<0.05).Compared with those in the electroacupuncture group,the horizontal motor score,vertical mo-tor score and sugar water consumption of the rats in the inhibitor gro

关 键 词：慢性前列腺炎 电针 细胞凋亡 TLR4/NF-κB/NLRP3通路 

分 类 号：R245.31[医药卫生—针灸推拿学]