穿心莲内酯对胰岛素抵抗大鼠的影响及可能作用机制分析  

Effect of Andrographolide on Insulin Resistance Rats and Analysis of Its Possible Mechanisms of Action

作　　者：武莉[1] 冯吉波 王延茹 李锦平[1] WU Li;FENG Jibo;WANG Yanru;LI Jinping(Fenyang College,Shanxi Medical University,Fenyang 032200,Shanxi,China)

机构地区：[1]山西医科大学汾阳学院,山西汾阳032200

出　　处：《中华中医药学刊》2024年第9期246-250,I0050,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：山西省高等学校科技创新项目(2022L674);山西省教学改革科技创新项目(J20221396);山西医科大学汾阳学院大学生创新创业训练计划项目(FDC202118);山西医科大学汾阳学院科研项目(1426)。

摘　　要：目的 探讨穿心莲内酯对胰岛素抵抗大鼠的影响及可能作用机制。方法 获得SPF级健康成年Wistar大鼠48只,随机选择36只大鼠经高脂高糖饲料喂养建立实验性Wistar大鼠IR模型,按照随机数字分配法分为空白对照模型组(HFD组)、穿心莲内酯高剂量组(High组)和穿心莲内酯低剂量组(Low组),选择12只大鼠接受普通饲料喂养作为正常对照组(NC组),High组大鼠予以经腹腔注射穿心莲内酯200 mg/(kg·d),Low组大鼠予以穿心莲内酯腹腔注射100 mg/(kg·d),HFD组以及NC组仅予以等量生理盐水腹腔注射,连续14 d。应用葡萄糖氧化酶法对空腹血糖值(FBG)水平进行监测,应用ELISA法检测空腹胰岛素(Fasting insulin, FINS),计算稳态模型评估胰岛素抵抗指数(homeostasis model assessment-insulin resistance, HOMA-IR);油红O染色制片检测肝脏的病理变化;应用联苯三酚自氧化法对超氧化物歧化酶(superoxide dismutase, SOD)活力,应用硫代巴比妥酸法对丙二醛(malondialdehyde, MDA)水平进行监测;免疫组化法检测大鼠肝脏组织内核糖体蛋白S6激酶(Ribosomal protein S6 kinase, S6K1)及p-胰岛素受体底物-1(p-insulin receptor substrate-1,p-IRS-1)(Tyr632)蛋白表达水平,Westernblotting法检测肝脏组织内S6K1及p-IRS-1(Tyr632)表达水平。应用SPSS 23.0软件包进行分析。结果 相比NC组比较,HFD组、High组、Low组大鼠FBG、FINS、HOMA-IR水平明显升高(P<0.05);与HFD组相比,其余3组FBG、FINS、HOMA-IR水平较低(P<0.05);与穿心莲内酯Low组相比,High组FBG、FINS、HOMA-IR水平较低(P<0.05)。相比NC组,HFD组光密度平均值增加(P<0.05);相比HFD组,穿心莲内酯High组与Low组脂滴面积/肌纤维总面积、光密度平均值均下降(P<0.05);与穿心莲内酯Low组相比,High组脂滴面积/肌纤维总面积、光密度平均值较低(P<0.05)。与NC组相比,HFD组SOD活性与MDA含量增加(P<0.05);相比HFD组,穿心莲内酯High组与Low组SOD活性与MDA含量均下降(P<0.05);与�Objective To investigate the effect and possible mechanism of andrographolide on insulin resistance in rats.Method A total of 48 healthy adult Wistar rats with SPF grade were obtained,and 36 rats were randomly fed with high-fat and high-sugar diets to establish an experimental Wistar rat insulin resistance(IR)model.The rats were divided into blank control model group(HFD group),androlianolide high-dose group(High group)and androlianolide low-dose group(Low group)according to random number allocation method.Twelve rats were selected to receive normal diet as the normal control group(NC group),High group was given intraperitoneal injection of andrographolide 200 mg/(kg·d).Low group was given intraperitoneal injection of andrographolide 100 mg/(kg·d).HFD group and NC group were only given intraperitoneal injection of the same amount of nor-mal saline for 14 consecutive days.Fasting blood glucose(FBG)was monitored by glucose oxidase method,and fasting insulin(FINS)was detected by ELISA method.The homeostasis model assessment-insulin resistance(HOMA-IR)was calculated.The pathological changes of liver were detected by red O staining.The activity of superoxide dismutase(SOD)was measured by biphloroglucinol autoxidation method,and malondialdehyde(MDA)level was measured by thiobarbituric acid method.Immuno-histochemical detection of ribosomal protein S6 kinase in rat liver tissue(ribosomal protein S6 kinase,S6K1)and p-insulin re-ceptor substrate-1(p-IRS-1)(Tyr632)protein expression levels.Western blotting was used to detect expression levels of S6K1 and p-IRS-1(Tyr632)in liver tissues.The SPSS 23.0 software package was used for analysis.Result Compared with those of the NC group,the levels of FBG,FINS and HOMA-IR in the HFD group,High group and Low group were significantly increased(P<0.05).Compared with the HFD group,the other three groups had lower levels of FBG,FINS and HOMA-IR(P<0.05).Compared with the Low group,the High group had lower levels of FBG,FINS and HOMA-IR(P<0.05).Com-pared with that of the NC group,the a

关 键 词：穿心莲内酯 胰岛素抵抗大鼠 氧化应激 S6K1表达 IRS-1酪氨酸磷酸化水平 

分 类 号：R282.71[医药卫生—中药学]