不同日龄坏死性小肠结肠炎大鼠脑损伤差异研究  

Study on the Difference of Brain Injury in Rats with Necrotizing Enterocolitis at Different Ages

作　　者：李华渊 鲁梦雪 孙颖 汪景 卢燕云 国玉 张丹丹 包容[4] LI Huayuan;LU Mengxue;SUN Ying;WANG Jing;LU Yanyun;GUO Yu;ZHANG Dandan;BAO Rong(Cloud-Clone Corp.Wuhan,Wuhan 430056,China;Wuhan Cloud-Clone Diagnostic Reagents Institute,Wuhan 430056,China;USCN Life Science Inc.Wuhan,Wuhan 430056,China;the Center for Animal Experiment/Animal Biosafety Level-3 Laboratory,Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉云克隆科技股份有限公司,武汉430056 [2]武汉云克隆诊断试剂研究所有限公司,武汉430056 [3]武汉优尔生检测中心有限公司,武汉430056 [4]武汉大学动物实验中心/ABSL-Ⅲ实验室,武汉430071

出　　处：《实验动物科学》2024年第4期15-21,共7页Laboratory Animal Science

基　　金：“十四五”国家重点研发计划重点专项(2021YFF0702002)。

摘　　要：目的建立3种不同日龄大鼠坏死性小肠结肠炎(NEC)模型,研究大鼠脑损伤差异。方法将60只SD乳鼠分为6组:1日龄对照组、1日龄模型组、3日龄对照组、3日龄模型组、5日龄对照组和5日龄模型组,每组10只。所有模型组大鼠采用人工喂养、缺氧、冷刺激和脂多糖(LPS)诱导建立NEC模型,对照组大鼠均鼠乳喂养及正常环境饲养。建模期间观察大鼠活动、腹胀、腹泻等情况,并监测体质量变化。大鼠处死后,观察肠组织整体形态。HE染色观察小肠组织病理变化,并进行损伤评分。尼氏染色观察脑组织尼氏体数量变化。TUNEL染色观察脑组织细胞凋亡水平。结果与相同日龄对照组比较,模型组大鼠出现腹胀、腹泻情况,活动度差,体质量明显减轻(P<0.05),肠组织出现肿胀、积气和坏死灶,小肠组织病理损伤严重,损伤评分显著升高(P<0.05),脑组织尼氏体数量显著减少(P<0.05),而脑组织细胞凋亡增多,且1日龄NEC大鼠肠组织和脑组织损伤最严重。结论不同日龄NEC大鼠之间肠组织和脑组织损伤存在差异,日龄越小,损伤程度越严重,提示日龄可能是NEC大鼠脑损伤的危险因素之一。Objective Three models of necrotizing enterocolitis(NEC)in rats of different ages were established to study the differences of brain injury.Method Sixty newborn SD rats were divided into 6 groups:1-day-old control group,1 day old model group,3 day old control group,3 day old model group,5 day old control group and 5 day old model group,with 10 rats in each group.NEC models were established in all model groups by artificial feeding,hypoxia,cold stimulation and lipopolysaccharide(LPS)induction.Rats in the control group were fed with breast milk in normal environment.During the modeling period,the activity,abdominal distension and diarrhea of rats were observed,and the weight changes were monitored.After the rats were killed,the overall morphology of intestinal tissue was observed.HE staining was used to observe the pathological changes of small intestine tissue,and the injury score was performed.Nissl staining was used to observe the number of nissl bodies in brain tissue.TUNEL staining was used to observe the level of apoptosis in brain tissue.Result Compared with the control group at the same age,the rats in the model group had abdominal distension,diarrhea,poor activity,the weight decreased significantly(P<0.05),swelling,gas accumulation and necrotic foci in the intestinal tissue.The pathological damage of small intestinal tissue was serious,the injury score was significantly increased(P<0.05),the number of Nissi bodies in brain tissue was significantly decreased(P<0.05),and the cell apoptosis in the brain tissue was increased.Moreover,the intestinal tissue and brain tissue injuries of 1-day-old NEC rats were the most serious.Conclusion There are differences in intestinal and brain tissue injuries among NEC rats at different ages.The younger the age,the more severe the injury,suggesting that the age of onset may be one of the risk factors for brain injury in NEC rats.

关 键 词：坏死性小肠结肠炎 大鼠 日龄 脑损伤 

分 类 号：Q95-3[生物学—动物学]