Mild synthesis of ultra-bright carbon dots with solvatochromism for rapid lipid droplet monitoring in varied physiological processes  

作　　者：Borui Su Dong Gao Nini Xin Kai Wu Mei Yang Shichao Jiang Yusheng Zhang Jie Ding Chengheng Wu Jing Sun Dan Wei Hongsong Fan Zhenzhen Guo 

机构地区：[1]National Engineering Research Center for Biomaterials,College of Biomedical Engineering,Sichuan University,Chengdu,Sichuan 610064,China [2]Institute of Regulatory Science for Medical Devices,Sichuan University,Chengdu,Sichuan 610064,China [3]Department of Gastroenterology,Sichuan Provincial People's Hospital,University of Electronic Science and Technology of China,Chengdu 610o72,China

出　　处：《Regenerative Biomaterials》2024年第3期30-42,共13页再生生物材料（英文版）

基　　金：the National Natural Science Foundation of China(grant numbers:52003178 and 51973132);Intermnational Science and Technology Innovation Cooperation Foundation of Sichuan Province(grant number:2022YFH0086);Natural Science Foundation of Sichuan Province(grant number:2023NSFSC0338 and 2023NSFSC1067).

摘　　要：Lipid droplets(LDs)participating in various cellular activities and are increasingly being emphasized.Fluorescence imaging provides powerful tool for dynamic tracking of LDs,however,most current LDs probes remain inconsistent performance such as low Photoluminescence Quantum Yield(PLQY),poor photostability and tedious washing procedures.Herein,a novel yellow-emissive carbon dot(OT-cD)has been synthesized conveniently with high PLQY up to 90%.Besides,OT-CD exhibits remarkable amphiphilicity and solvatochromic property with lipid-water partition coefficient higher than 2,which is much higher than most LDs probes.These characters enable OT-CD high brightness,stable and wash-free LDs probing,and feasible for in vivo imaging.Then,detailed observation of LDs morphological and polarity variation dynamically in different cellular states were recorded,including ferroptosis and other diseases processes.Furthermore,fast whole imaging of zebrafish and identifed LD enrichment in injured liver indicate its further feasibility for in vivo application.In contrast to the reported studies to date,this approach provides a versatile conventional synthesis system for high-performance LDs targeting probes,combing the advantages of easy and high-yield production,as well as robust brightness and stability for long-term imaging,facilitating investigations into organelle interactions and LD-associated diseases.

关 键 词：lipid droplet probe carbon dots fluorescence imaging wash-free dynamic monitoring 

分 类 号：R318[医药卫生—生物医学工程]