Construction of millimeter-scale vascularized engineered myocardial tissue using a mixed gel  

作　　者：Ming Ke Wenhui Xu Yansha Hao Feiyang Zheng Guanyuan Yang Yonghong Fan Fangfang Wang Zhiqiang Nie Chuhong Zhu 

机构地区：[1]Department of Anatomy,Third Military Medical University,Chongqing 400038,China [2]State Key Laboratory of Trauma,Burn and Combined Injury,Chongqing 400038,China [3]Department of Plastic and Aesthetic Surgery,Southwest Hospital,Third Military Medical University,Chongqing 400038,China [4]Engineering Research Center of Tissue and Organ Regeneration and Manufacturing,Ministry of Education,Chongqing 400038,China

出　　处：《Regenerative Biomaterials》2024年第3期56-67,共12页再生生物材料（英文版）

基　　金：Key projects of the National Natural Science Foundation of China(81830055,82230073);the Postdoctoral Research Special Support Projects of Chongqing(2020-2010010006263477);Thanks for the support of Yonghong Fan's the Science and Technology Innovation Enhancement Projects of Third Military Medical University(2020xQN03).

摘　　要：Engineering myocardium has shown great clinal potential for repairing permanent myocardial injury.However,the lack of perfusing blood vessels and difficulties in preparing a thick-engineered myocardium result in its limited clinical use.We prepared a mixed gel containing fibrin(5 mg/ml)and collagen I(0.2 mg/ml)and verified that human umbilical vein endothelial cells(HUVECs)and human-induced pluripotent stem cell-derived cardiomyocytes(hiPSC-CMs)could fom microvascular lumens and myocardial cell clusters by harnessing the low-hardness and hyperelastic characteristics of fibrin.hiPSC-CMs and HUVECs in the mixed gel formed self-organized cell clusters,which were then cultured in different media using a three-phase approach.The successfully constructed vascularized engineered myocardial tissue had a spherical structure and final diameter of 1-2mm.The tissue exhibited autonomous beats that occured at a frequency similar to a normal human heart rate.The internal microvascular lumen could be maintained for 6 weeks and showed good results during preliminary surface re-vascularization in vitro and vascular remodeling in vivo.In summary,we propose a simple method for constructing vascularized engineered myocardial tissue,through phased cultivation that does not rely on high-end manufacturing equipment and cutting-edge preparation techniques.The constructed tissue has potential value for clinical use after preliminary evaluation.

关 键 词：engineered myocardium vascularized hiPSC-CM HUVEC FIBRIN 

分 类 号：R318[医药卫生—生物医学工程] TB383[医药卫生—基础医学]