Enhancing cartilage regeneration and repair through bioactive and biomechanical modification of 3D acellular dermal matrix  

作　　者：Wei Gao Tan Cheng Zhengya Tang Wenqiang Zhang Yong Xui Min Han Guangdong Zhou Chunsheng Tao Ning Xu Huitang Xia Weijie Sun 

机构地区：[1]Qingdao Medical College of Qingdao University,Qingdao,266071,China [2]Department of Infectious Diseases,The First Affliated Hospital of Anhui Medical University,Shushan,Hefei,230022,China [3]Department of Orthopaedic Surgery,Shanghai Sixth People's Hospital Affliated to Shanghai jiao Tong University School of Medicine,Shanghai,200025,China [4]Department of Orthopedic Surgery,Shanghai Eighth People's Hospital,Shanghai,200235,China [5]Department of PlasticSurgery&Jinan ClinicalResearch Center for Tissue Engineering Skin Regeneration and Wound Repair,The First Affliated Hospital of Shandong First Medical University,Jinan,266299,China [6]Department of Orthopaedics,Ninety-seventh Hospital of the Chinese People's Liberation Army Navy,Qingdao,266071,China [7]Department of Orthopaedics,The First Affliated Hospital of Shandong First Medical University,Jinan,266299,China [8]Department of Plastic surgery,Shanghai Key Laboratory of Tissue Engineering,Shanghai Ninth People's Hospital,Shanghai jiao Tong University School of Medicine,Shanghai,200023,China [9]Department of Thoracic Surgery,Shanghai Children's Hospital,Shanghai Jiao Tong University School of Medicine,Shaghai,200040,China [10]Department of Thoracic Surgery,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,200433,China

出　　处：《Regenerative Biomaterials》2024年第3期105-121,共17页再生生物材料（英文版）

基　　金：the National Natural Science Foundation of China(82302395,82102348,82001979,82372390 and 31900963);the Natural Science Foundation of Shanghai(22YF1437400);Young Elite Scientists Sponsorship Program by CAST(2023QNRC001);the Health-Education Joint Research Project of Fujian Province(2019-WJ-22);Taishan Scholar Program of Shandong Province(tsqn20230633);ShanghiaHi ealth Promotion Commission,Shanghai 2023 Health Science Popularization Special Plan‘Prevention and Control Science Popularization System for Hip Fall Injury in the Elderly'(JKKPZX-2023-A27)and the Fundamental Research Funds for the Central Universities(2021CDJQY-017).

摘　　要：Acellular dermal matrix(ADM)shows promise for cartilage regeneration and repair.However,an effective decellularization technique that removes cellular components while preserving the extracellular matrix,the transformation of 2D-ADM into a suitable 3D scaffold with porosity and the enhancement of bioactive and biomechanical properties in the 3D-ADM scaffold are yet to be fully addressed.In this study,we present an innovative decellularization method involving 0.125%trypsin and 0.5%SDS and a 1%Triton X-100 solution for preparing ADM and converting 2D-ADM into 3D-ADM scaffolds.These scaffolds exhibit favorable physicochemical properties,exceptional biocompatibility and significant potential for driving cartilage regeneration in vitro and in vivo.To further enhance the cartilage regeneration potential of 3D-ADM scaffolds.we incorporated porcine-derived small intestinal submucosa(SIS)for bioactivity and calcium sulfate hemihydrate(CSH)for biomechanical reinforcement.The resulting 3D-ADM+SIS scaffolds displayed heightened biological activity,while the 3D-ADM+CSH scaffolds notably bolstered biomechanical strength.Both scaffold types showed promise for cartilage regeneration and repair in vitro and in vivo,with considerable improvements observed in repairing cartilage defects within a rabbit articular cartilage model.In summary,this research introduces a versatile 3D-ADM scaffold with customizable bioactive and biomechanical properties,poised to revolutionize the field of cartilageregeneration.

关 键 词：acellular dermal matrix three-dimensional scaffolds cartilage regeneration small intestinal submucosa calcium sulfate hemihydrate 

分 类 号：R318[医药卫生—生物医学工程]