普氏原羚与放牧绵羊的粪便细菌多样性差异研究  

Preliminary Study on Differences in Bacterial Diversity in Feces of Przewalski's Gazelle and Grazing Sheep

作　　者：李生庆[1,2] 郭文涛 胡国元[1,2] 李淑萍[1,2] 韩生义 王威阳 LI Shengqing;GUO Wentao;HU Guoyuan;LI Shuping;HAN Shengyi;WANG Weiyang(Academy of Animal and Veterinary Sciences,Qinghai University,Xining 810016,China;Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and GreenTechnical Research for Prevention and Control,Xining 810016,China;Qinghai institute for endemic disease Prevention and Control,Xining 811602,China;College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学畜牧兽医科学院,青海西宁810016 [2]青海省动物疾病病原诊断与绿色防控技术研究重点实验室,青海西宁810016 [3]青海省地方病预防控制所,青海西宁811602 [4]青海大学农牧学院,青海西宁810016

出　　处：《青海畜牧兽医杂志》2024年第4期28-31,40,共5页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：青海省科技厅自然科学基金面上项目(2021-ZJ-923)。

摘　　要：采用16S rDNA技术对刚察县、海晏县的普氏原羚(Procapra przewalskii)和刚察县放牧绵羊的粪便进行细菌多样性的初步分析,结果表明,在门分类水平上,海晏、刚察两个地区的普氏原羚及放牧绵羊的肠道微生物群落主要分布为以下6个优势菌门(占比大于1%),包括厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、疣微菌门(Verrucomicrobia)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)和螺旋体门(Spirochaetes)等,表明两种动物在肠道微生物多样性上具有一致性。此外,普氏原羚肠道中放线菌门相对丰度占比高于放牧绵羊,且差异极显著(P<0.01),而放牧绵羊肠道中疣微菌门相对丰度占比极显著高于普氏原羚肠道中的微生物含量。This study used 16S rDNA technology to analyze the Przewalski's gazelle(Procapra Przewalsk II)in Gangcha County.Preliminary analysis of bacterial diversity in the feces of grazing sheep showed that:at the phylum level,the intestinal microbial communities of Przewalski's gazelle and grazing sheep in Haiyan and Gangcha were mainly distributed in the following 6 dominant bacterial phyla(proportion of more than 1%),including Firmicutes,Bacteroidetes,Verrucomicrobia,Actinobacteria,Proteobacteria,Spirochaetes,etc.It indicated that there was a certain consistency in gut microbial diversity between the two animals.The correlation difference analysis of the content of Verrucomicrobia,Actinomycetes,Proteobacteria,and Spirochetes in the intestinal microbes of Przewalski's gazelle and grazing sheep showed that Actinobacteria in the Przewalski's gazelle intestinal tract The proportion of verrucous microbes in the gut of grazing sheep was significantly higher than that of grazing sheep,and the difference was extremely significant(P<0.01).

关 键 词：普氏原羚 放牧绵羊 菌群多样性 16S rRNA 

分 类 号：S852.6[农业科学—基础兽医学]