PB设计-响应面优化橘绿木霉菌降解山西褐煤的工艺条件  

Optimization of technological conditions for the degradation of Shanxi lignite by Trichoderma citrinoviride with PB design-response surface

作　　者：沈晖 冯炘[1] 李梅彤 袁文蛟 解玉红[1,2] SHEN Hui;FENG Xin;LI Meitong;YUAN Wenjiao;XIE Yuhong(School of Environmental Science and Safety Engineering,Tianjin University of Technology,Tianjin 300384,China;Easy Clear(Tianjin)Environmental Protection Science&Technology Co.,Ltd.,Tianjin 300391,China)

机构地区：[1]天津理工大学环境科学与安全工程学院,天津300384 [2]易科力(天津)环保科技发展有限公司,天津300391

出　　处：《天津理工大学学报》2024年第4期1-9,共9页Journal of Tianjin University of Technology

基　　金：天津市科技计划项目(22YDTPJC00760,18ZXSZSF00090);天津市技术创新引导专项(20YDTPJC01790);天津市重点研发计划(21YFSNSN00190)。

摘　　要：以实验室从银耳种子中筛选出的橘绿木霉菌(Trichoderma citrinoviride)为实验菌株,以山西褐煤为实验材料,以山西褐煤的降解率为指标,优化橘绿木霉菌降解山西褐煤的工艺条件。在单因素试验的基础上,运用Plackett-Burman设计方法,筛选出对山西褐煤降解率具有显著影响的因素,最后运用Box-Behnken响应面试验建立各具有显著影响的因素之间的二次回归方程模型,从而得到理论上的橘绿木霉菌降解山西褐煤的最佳工艺条件。最佳降解工艺条件为:环境温度28.4℃,环境pH值6.48,接菌量6%(V/V),煤样粒度120~150目,煤浆浓度2.025 g·100 mL^(-1),降解时间24天。在该工艺条件之下,山西褐煤的降解率为(44.34±0.47)%。Taking Trichoderma citrinoviride,which was screened out from Tremella seeds in the laboratory,as the experimental strain,taking Shanxi lignite as the experimental material,taking the degradation rate of Shanxi lignite as an indicator to optimize the technological conditions for the degradation of Shanxi lignite by Trichoderma citrinoviride to improve the degradation rate.On the basis of the single factor test,the Plackett-Burman test was used to screen out the factors which have a significant impact on the degradation rate of Shanxi lignite.Finally,the Box-Behnken response surface test was used to establish the quadratic regression equation model between the factors with significant influence,so as to obtain the theoretical optimum technological conditions for the degradation of Shanxi lignite by Trichoderma citrinoviride.The optimum degradation process conditions are:ambient temperature 28.4℃,ambient pH value 6.48,inoculation volume 6%(V/V),coal sample particle size 120 mesh-150 mesh,coal slurry concentration 2.025 g·100 mL^(-1),degradation time 24 days.Under this process conditions,the degradation rate of Shanxi lignite was(44.34±0.47)%.

关 键 词：橘绿木霉菌 山西褐煤 降解工艺 响应面法 

分 类 号：TD849.2[矿业工程—煤矿开采]