丝素/Ⅱ型胶原复合支架降解过程中的微观结构研究  

Study on microstructure of filament/type Ⅱ collagen composite scaffold during degradation process

作　　者：颜研 高丽兰[1,2] 魏莹 谭沿松 张春秋[1,2] YAN Yan;GAO Lian;WEI Ying;TAN Yansong;ZHANG Chunqu(Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control,Tianjin University of Technology,Tianjin 300384,China;National Demonstration Center for Experimental Mechanical and Electrical Engineering Education,Tianjin University of Technology,Tianjin 300384,China)

机构地区：[1]天津理工大学天津市先进机电系统设计与智能控制重点实验室,天津300384 [2]天津理工大学机电工程国家级实验教学示范中心,天津300384

出　　处：《天津理工大学学报》2024年第4期98-103,共6页Journal of Tianjin University of Technology

基　　金：国家自然科学基金面上项目(32271371);天津市自然科学多元投入基金面上项目(21JCYBJC00940,21JCQNJC01310)。

摘　　要：理想的软骨组织工程支架不仅要具备良好的力学性能、生物相容性以及空间结构,生物可降解性也是支架临床应用重点研究的对象。支架降解过快或过慢都会对组织的再生速率产生影响。文中采用红外光谱法研究处于不同灌流速度刺激下的丝素蛋白/Ⅱ型胶原复合支架降解时蛋白二级结构的变化。结果表明:相同降解时间下,灌流速度越大,该复合材料支架蛋白二级结构变化越明显,其中β-sheet和α-form结构的含量均呈现出先快后慢的增加趋势,而无规则卷曲结构含量则表现为下降趋势。因此,该复合支架为制备降解速率可调控的生物材料提供了可行性且具有一定临床指导意义。The ideal cartilage tissue engineering scaffold has not only good mechanical properties,biocompatibility and spatial structure,biodegradability is also the key research target for clinical applications of the scaffold.Too fast or too slow degradation of the stent can have an impact on the rate of tissue regeneration.In this paper,infrared spectroscopy was used to study the changes in the secondary structure of protein when the silk protein/type Ⅱ collagen composite scaffold is degraded under the different perfusion rates.The results show that the greater the perfusion rate under the same degradation time,the more obvious the secondary structure of the scaffold protein is changed.The contents of β-sheet and α-form structure increase fast and then slowly,while the content of irregular curl structure shows a downward trend.This composite scaffold provides feasibility as the biomaterials with adjustable degradation rate and has certain clinical guidance significance.

关 键 词：软骨支架 灌流降解 力学刺激 微观结构 红外光谱 

分 类 号：R318.08[医药卫生—生物医学工程]