枯草芽孢杆菌复合制剂对蛋鸡生产性能和肠道菌群的影响  

Effect of Bacillus subtilis compound preparation on production performance and gut microbiota of laying hens

作　　者：付强[1] 郭时金[1] 王建军[1] 程立坤[1] 赵修报 姜忠兴 付石军 FU Qiang;GUO Shi-jin;WANG Jian-jun;CHENG Li-kun;ZHAO Xiu-bao;JIANG Zhong-xing;FU Shi-jun

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东绿都生物科技有限公司,山东滨州256600

出　　处：《饲料研究》2024年第15期34-39,共6页Feed Research

基　　金：山东省重点研发计划项目(项目编号:2019GNC106067);山东省中央引导地方科技发展资金项目(项目编号:YDZX2023015);滨州市农社领域科技创新政策引导计划项目(项目编号:2022KTPY009)。

摘　　要：试验旨在探究枯草芽孢杆菌复合制剂对蛋鸡生产性能和肠道菌群的影响。选取30只500日龄体重相近的蛋鸡随机分为2组,每组3个重复,每个重复5只蛋鸡。CK组(对照组)饲喂玉米-豆粕基础饲粮,T组在基础饲粮中添加1 g/kg枯草芽孢杆菌复合制剂。试验期70 d。结果显示,与CK组相比,T组料蛋比显著降低(P<0.05);在门水平,十二指肠厚壁菌门相对丰度极显著增加(P<0.01),回肠放线菌门相对丰度显著增加(P<0.05),盲肠变形菌门相对丰度极显著降低(P<0.01);在属水平,十二指肠乳杆菌属相对丰度极显著增加(P<0.01),棒状杆菌属丰度极显著降低(P<0.01),回肠肠球菌属和卡多维亚氏菌(Aeriscardovia)相对丰度显著或极显著增加(P<0.05或P<0.01),盲肠理研菌科相对丰度显著增加(P<0.05)。β多样性分析显示,十二指肠和回肠菌群结构具有一定的相似性,且与盲肠菌群结构不同。研究表明,添加枯草芽孢杆菌复合制剂可促进乳酸菌、Aeriscardovia生长,优化了肠道菌群结构,提高了饲料利用率。The experiment aimed to explore the effects of a Bacillus subtilis compound preparation on the production performance and intestinal microbiota of laying hens.Thirty 500-day-old hens with similar body weights were randomly divided into two groups,with three replicates per group,and each replicate consisted of five hens.The CK group(control group)was fed a corn-soybean meal basic diet,while the T group was supplemented with 1 g/kg Bacillus subtilis compound preparation in the basic diet.The trial lasted for 70 days.The results showed that compared with the CK group,the feed-to-egg ratio in the T group was significantly reduced(P<0.05).At the phylum level,the relative abundance of the Firmicutes in the duodenum was extremely increased(P<0.01),the relative abundance of the Actinobacteria in the ileum was significantly increased(P<0.05),and the relative abundance of the Proteobacteria in the cecum was extremely decreased(P<0.01).At the genus level,the relative abundance of Lactobacillus in the duodenum was extremely increased(P<0.01),the abundance of Corynebacterium was extremely decreased(P<0.01),and the relative abundance of Enterococcus and Aeriscardovia in the ileum was significantly or extremely significantly increased(P<0.05 or P<0.01),and the relative abundance of the Ruminococcaceae in the cecum was significantly increased(P<0.05).β-diversity analysis showed that the microbial community structures of the duodenum and ileum had certain similarities and were different from the cecal microbial community structure.The study suggests that the addition of Bacillus subtilis compound preparation can promote the growth of lactic acid bacteria and Aeriscardovia,playing a role in optimizing the intestinal microbiota and improving feed utilization.

关 键 词：枯草芽孢杆菌复合制剂 蛋鸡 生长性能 肠道菌群 高通量测序 菌群多样性 

分 类 号：S831.5[农业科学—畜牧学]