炒白术对肠易激综合征小鼠血管内皮功能及血清Slit2表达的影响  

Effects of fried Baizhu on vascular endothelial function and serum Slit2 expression in mice with irritable bowel syndrome

作　　者：张一 潘晓艳 王秀苇 陈丽霞 谢烨婷 陈坚翱[1] 俞蕾敏 ZHANG Yi;PAN Xiaoyan;WANG Xiuwei;CHEN Lixia;XIE Yeting;CHEN Jianao;YU Leimin(Department of Gastroenterology,Hangzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University,Hangzhou,Zhejiang 310007,China;Traditional Chinese Medicine Dispensary,Hangzhou Shangcheng Xiaoying Street Community Health Service Center,Hangzhou,Zhejiang 310003,China;Department of School Hospital,Zhejiang Sci-Tech University,Hangzhou,Zhejiang 310018,China;School of Nurs ing,Zhejiang Chinese Medical University,Hangzhou,Zhejiang 310053,China)

机构地区：[1]浙江中医药大学附属杭州市中医院脾胃病科,杭州310007 [2]杭州市上城区小营街道社区卫生服务中心中药房,杭州310003 [3]浙江理工大学校医院,杭州310018 [4]浙江中医药大学护理学院,杭州310053

出　　处：《浙江中西医结合杂志》2024年第9期797-802,共6页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2023ZL101)。

摘　　要：目的 探讨炒白术通过影响神经生长导向因子2(Slit2)表达改善肠易激综合征(IBS)小鼠肠道血管内皮功能的作用机制。方法 50只正常雌性C57BL/6小鼠,随机选取10只作为对照组,其余采用束缚应激建立IBS小鼠模型,并随机分为模型组、低剂量白术组(0.05 g/mL)、中剂量白术组(0.1 g/mL)、高剂量白术组(0.5 g/mL),各10只。造模后,低、中、高剂量白术组予不同剂量炒白术煎剂灌胃治疗7 d,对照组和模型组予等量生理盐水灌胃,治疗结束后利用结直肠扩张-腹壁撤回反射(AWR)法评估小鼠内脏敏感性。酶联免疫吸附试验检测小鼠血清Slit2、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、诱导型一氧化氮合成酶(i NOS)、内皮素1(ET-1)含量,硝酸还原酶法检测小鼠血清一氧化氮(NO)含量,蛋白质印记检测小鼠肠道封闭蛋白1(claudin1)、紧密连接蛋白(occludin)含量。结果 与对照组比较,模型组小鼠AWR评分在直肠扩张压力40 mmHg(1 mmHg=0.133 kPa)[(2.31±0.49)分比(1.54±0.44)分,P<0.05]和60 mmHg[(3.43±0.55)分比(2.43±0.47)分,P<0.01]时显著升高,血清Slit2含量下降[(666.36±152.22)pg/mL比(923.06±146.23)pg/mL,P<0.01],IL-1β[(80.31±6.87)pg/mL比(41.95±7.64)pg/mL,P<0.01]、TNF-α[(81.17±13.62)pg/mL比(34.33±8.31)pg/mL,P<0.01]、i NOS [(17.70±1.85)ng/mL比(9.22±2.07)ng/mL,P<0.01]、ET-1 [(132.10±9.25)pg/mL比(89.52±6.85)pg/mL,P<0.01]、NO[(61.69±8.29)pg/mL比(31.02±6.24)pg/mL,P<0.01]含量均显著升高,肠道occludin[(0.89±0.09)比(1.08±0.10),P<0.05]、claudin1[(0.95±0.12)比(1.26±0.10),P<0.01]含量明显下降。与模型组比较,高剂量白术组AWR评分在直肠扩张压力40 mmHg[(1.64±0.41)分比(2.31±0.49)分,P<0.05]和60 mmHg[(2.76±0.50)分比(3.43±0.55)分,P<0.05]时均明显下降,血清Slit2含量显著升高[(943.76±182.17)pg/mL比(666.36±152.22)pg/mL,P<0.01],IL-1β [(64.81±8.53)pg/mL比(80.31±6.87)pg/mL,P<0.01]、TNF-α [(53.36±10.40)pg/mL比(81.17±13.62)pg/mL,P<0.01]、i NObjective To investigate the effect and mechanism of fried Baizhu on improving intestinal vascular endothelial function in mice with irritable bowel syndrome(IBS)by affecting Slit2 expression.Methods Fifty female C57BL/6 mice were randomly divided into a control group and four experimental groups:model,low-dose(0.05 g/mL),medium-dose(0.1 g/mL),and high-dose Baizhu(0.5 g/mL)groups,with 10 mice each.The experimental groups were subjected to restraint stress to induce an IBS model.After modeling,the low-,medium-and high-dose Baizhu groups were treated with different doses of fried Baizhu decoction by gavage for 7 days.The control and model groups were treated with equal volumes of saline.After the treatment,colorectal distention-abdominal withdrawal reflex(AWR)method was used to evaluate the visceral sensitivity of mice.Enzyme-linked immunosorbent assay was used to detect the levels of Slit2,interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),inducible nitric oxide synthase(iNOS),and endothelin-1(ET-1)in the mouse serum.The nitrate reductase method was used to detect the content of nitric oxide(NO)in the mouse serum.The contents of intestinal claudin1 and occludin were detected using western blotting.Results Compared with the control group,the model group showed significantly increased AWR scores at 40 mmHg(1 mmHg=0.133 kPa)of rectal dilation pressure[(2.31±0.49)vs.(1.54±0.44)points,P<0.05]and 60 mmHg[(3.43±0.55)vs.(2.43±0.47)points,P<0.01],and the decreased serum Slit2 level[(666.36±152.22)vs.(923.06±146.23)pg/mL,P<0.01].The model group also showed significantly increased serum levels of IL-1β[(80.31±6.87)vs.(41.95±7.64)pg/mL,P<0.01],TNF-α[(81.17±13.62)vs.(34.33±8.31)pg/mL,P<0.01],iNOS[(17.70±1.85)vs.(9.22±2.07)ng/mL,P<0.01],ET-1[(132.10±9.25)vs.(89.52±6.85)pg/mL,P<0.01],and NO[(61.69±8.29)vs.(31.02±6.24)pg/mL,P<0.01].However,the intestinal occludin[(0.89±0.09)vs.(1.08±0.10),P<0.05]and claudin1[(0.95±0.12)vs.(1.26±0.10),P<0.01]levels were significantly decreased.Compared with the model gr

关 键 词：小鼠 炒白术 肠易激综合征 血管内皮功能 肠黏膜屏障 SLIT2 

分 类 号：R285.5[医药卫生—中药学]