拟除虫菊酯农药类特异性传感体系构建及其在柑橘农残检测中的应用  

Pyrethroid pesticide-specific sensing system and its application in citrus pesticide residue detection

作　　者：黄欣娅 李泓霖 郑业坤 运欢 郭婷[1,2,3] 张宇昊 马良[1,2,3,4] HUANG Xinya;LI Honglin;ZHENG Yekun;YUN Huan;GUO Ting;ZHANG Yuhao;MA Liang(College of Food Science,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Specialty Food Co-Built by Sichuan and Chongqing,Chongqing 400715,China;Key Laboratory of Quality and Safety Control of Citrus Fruits,Ministry of Agriculture and Rural Affairs,Southwest University,Chongqing 400715,China;Key Laboratory of Condiment Supervision Technology for State Market Regulation,Chongqing 400715,China)

机构地区：[1]西南大学食品科学学院,重庆400715 [2]川渝共建特色食品重庆市重点实验室,重庆400715 [3]农业农村部柑橘类果品质量安全重点实验室,重庆400715 [4]国家市场监管重点实验室(调味品监管技术),重庆400715

出　　处：《食品与发酵工业》2024年第17期128-138,共11页Food and Fermentation Industries

基　　金：重庆市市场监督管理局科研计划项目(CQSJKJ2021016);重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0371);国家自然科学基金项目(32102062)。

摘　　要：拟除虫菊酯类农药在我国柑橘等果品中的施用量占全球第一,对其残留的快速筛查和监管对于保障我国果品食用安全具有重要意义。该文研究多功能纳米材料(NaYF_(4)∶Yb,Er-Fe_(3)O_(4)@MIP)的制备条件,采用傅里叶变换红外光谱、透射电子显微镜对该材料进行表征,通过对NaYF_(4)∶Yb,Er-Fe_(3)O_(4)@MIP的吸附和选择性实验,验证了其对4种拟除虫菊酯类农药具有较好的选择性和吸附性能,并构建基于该材料的拟除虫菊酯农药类特异性传感体系对柑橘果品菊酯类农残进行快速检测。结果表明,该传感体系在0.02~2.5 mg/L内对拟除虫菊酯类农药(溴氰菊酯、氯氰菊酯、氟氯氰菊酯、氰戊菊酯)具有良好的线性关系,检出限分别为6.17×10^(-4)、6.65×10^(-4)、7.21×10^(-4)、9.61×10^(-4)、6.43×10^(-4)mg/L。结合柑橘鲜果专属前处理技术进行实际柑橘样品检测,加标回收率为87.9%~105.8%,相对标准偏差为0.53%~6.41%,方法准确、灵敏、稳定,可以用于实际柑橘样品中拟除虫菊酯类农药的检测。The amount of pyrethroid pesticides used in citrus and other fruits in China ranks first in the world.Rapid screening and supervision of their residues are of great significance to ensure the safety of China’s fruits.This article studied the preparation conditions of multifunctional nanomaterials(NaYF_(4)∶Yb,Er-Fe_(3)O_(4)@MIP),and used Fourier transform infrared spectroscopy and transmission electron microscopy to characterize the material.Through the adsorption and selectivity experiments of NaYF_(4)∶Yb,Er-Fe_(3)O_(4)@MIP,it was verified that NaYF_(4)∶Yb,Er-Fe_(3)O_(4)@MIP had good selectivity and adsorption performance for four kinds of pyrethroid pesticides.Based on the above results,a pyrethroid pesticide-specific sensing system based on this material was constructed to rapidly detect pyrethroid pesticide residues in citrus fruits.Results showed that the sensing system had a good linear relationship with pyrethroid pesticides(deltamethrin,cypermethrin,cyfluthrin,and fenvalerate)in the range of 0.02-2.5 mg/L,and the detection limits were respectively 6.17×10^(-4),6.65×10^(-4),7.21×10^(-4),9.61×10^(-4),6.43×10^(-4)mg/L.Combined with the exclusive pre-treatment technology for fresh citrus fruits,actual citrus samples were tested.The spiked recovery rate was 87.9%-105.8%,and the relative standard deviation was 0.53%-6.41%.The method is accurate,sensitive and stable,and can be used for the detection of pyrethroid pesticides in actual citrus samples.

关 键 词：菊酯类农药 农残 上转换 荧光特性 复合纳米材料 柑橘 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]