基于便携GC-MS和HS/SPME-GC×GC-TOF MS分析化州橘红与广西橘红成分差异  

Analysis of Composition Differences between Huazhou Citri Grandis Exocarpium and Guangxi Citri Grandis Exocarpium Based on Portable GC-MS and HS/SPME-GC×GC-TOF MS

作　　者：李凯旋 黄罗旭 吴曼曼 徐经纬 谭国斌 罗思凡 周振 LI Kai-xuan;HUANG Luo-xu;WU Man-man;XU Jing-wei;TAN Guo-bin;LUO Si-fan;ZHOU Zhen(Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution,Institute of Mass Spectrometry and Atmospheric Environment,Jinan University,Guangzhou 510632,China;Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control,State Key Laboratory of Luminescent Materials and Devices,School of Environment and Energy,South China University of Technology,Guangzhou 510006,China;Guangdong MS Institute of Scientific Instrument Innovation,Guangzhou 510530,China;Guangzhou Hexin Analytical Instrument Company Limited,Guangzhou 510530,China)

机构地区：[1]暨南大学质谱仪器与大气环境研究所,广东省大气污染在线源解析系统工程技术研究中心,广东广州510632 [2]华南理工大学环境与能源学院,发光材料与器件国家重点实验室,广东省大气环境与污染控制重点实验室,广东广州510006 [3]广东省麦思科学仪器创新研究院,广东广州510530 [4]广州禾信仪器股份有限公司,广东广州510530

出　　处：《质谱学报》2024年第5期631-639,共9页Journal of Chinese Mass Spectrometry Society

基　　金：广州开发区科技计划项目(2020GH16)。

摘　　要：为探究不同产地橘红挥发性成分之间的差异,本研究采用便携气相色谱-质谱(GC-MS)和顶空固相微萃取结合全二维气相色谱-飞行时间质谱(GC×GC-TOF MS)技术分析化州橘红和广西橘红的挥发性成分。结果表明,便携GC-MS只能初步区分化州橘红和广西橘红,不能确定化州橘红的特征标志物;利用主成分分析法(PCA)和正交偏最小二乘法(OPLS-DA)对GC×GC-TOF MS实验数据进行处理,根据|p(corr)|>9和|p|>0.06结合VIP>1.1,共筛选出10种化州橘红标志物。该研究为化州橘红药材的质量评价与鉴定提供了依据,也为其他中药质量评价提供了方法参考。The volatile components of Citri Grandis Exocarpium from Huazhou(CGEH)and Guangxi(CGEG)were analyzed by portable gas chromatography-mass spectrometry(GC-MS)and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry(GC×GC-TOF MS)combined with headspace solid-phase microextraction(HS/SPME).Due to its limited separation ability,the portable GC-MS can identify only 8 chemicals in CGEH and 4 chemicals in CGEG and is not able to determine any marker compounds which can distinguish CGEH over CGEG.While the merit of GC×GC-TOF MS is strong separation ability,allowing the identification of 304 chemicals in CGEH and CGEG,of which 261 chemicals are reported for the first time.Among them are 52 compounds only detected in CGEH,81 ones unique in CGEG,and 171 detected in both CGEH and CGEG.The volatile components in CGEH and CGEG are classified into 17 and 19 types,respectively.The contents of alkene aromatics,hydrocarbons and alcohols in both CGEH and CGEG are relatively high.The principal component analysis(PCA)and orthogonal partial least squares(OPLS-DA)were further performed on the MS data obtained by GC×GC-TOF MS.The results indicated that there is basically no difference from different batches of fruits in the same region,while have obvious differences between CGEH and CGEG.Among these different components,10 compounds including 3,4-diethyl-1,1'-biphenyl,1-methyl-4-(1-methylethenyl)-1,2-cyclohexanediol,1-dodecanol,α-ethylidene-benzeneacetaldehyde,3,8-dimethyl-undecane,tetradecanal,2-methyl-tridecane,5-methyl-2(5H)-furanone,tetraethylene glycol,sabinol in CGEH are screened out with the values of Pearson correlation coefficient|p(corr)|>0.9,|p|>0.06 and variable influence on projection(VIP)value>1.1.On the other hand,several marker compounds,such as cedrane,hibaene,1,3,5-tris(methylene)-cycloheptane,(E)-longipinane,1,1,6,6-tetramethylspiro[4.4]nonane,2-(4a,8-dimethyl-1,2,3,4,4a,8a-hexahydro-2-naphthalenyl)-2-propanol,γ-gurjunene,[1S-(1α,4α,7α)]-1,2,3,4,5,6,7,8-octahydro-1,4,9,9-tetramethyl

关 键 词：橘红 便携气相色谱-质谱 全二维气相色谱-飞行时间质谱(GC×GC-TOF MS) 顶空固相微萃取(HS/SPME) 化学计量学分析 

分 类 号：O657.63[理学—分析化学]