蛇床子素通过阻断钙通道降低蛙坐骨神经复合动作电位幅度  

Osthole Attenuates Compound Action Potential Amplitude of Frog Sciatic Nerve by Inhibiting Calcium Channel

作　　者：李如廷 韦慧芳 覃翊杰 熊柳淇 沈宝如 王运踊 黄东湄 黄婕 何爽 伍冠一 LI Ruting;WEI Huifang;QIN Yijie;XIONG Liuqi;SHEN Baoru;WANG Yunyong;HUANG Dongmei;HUANG Jie;HE Shuang;WU Guanyi(College of Basic Medicine,Guangxi University of Chinese Medicine,Nanning 530299,Guangxi,China;Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine,Nanning 530299,Guangxi,China;Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models of Guangxi,Nanning 530299,Guangxi,China)

机构地区：[1]广西中医药大学基础医学院,广西南宁530299 [2]广西高发传染病中西医结合转化医学重点实验室,广西南宁530299 [3]广西特色实验动物病证模型重点实验室,广西南宁530299

出　　处：《辽宁中医杂志》2024年第9期134-136,I0002-I0004,共6页Liaoning Journal of Traditional Chinese Medicine

基　　金：广西自然科学基金项目(2020GXNSFAA297244);国家自然科学基金项目(81860740,82060768);研究生教育创新计划项目(YCSW2023392)。

摘　　要：目的探讨蛇床子素对蛙坐骨神经复合动作电位的作用以及离子机制。方法通过细胞外空气间隔法诱导蛙坐骨神经复合动作电位,观察蛇床子素作用及其机制。结果蛇床子素剂量依赖降低蛙坐骨神经复合动作电位幅度。胞外0钙能够减小蛇床子素的抑制效应。广谱钙通道阻断剂氯化镉,氯化镧阻断蛇床子素的抑制效应。L型钙通道阻断剂地尔硫卓、维拉帕米,P/T型钙通道阻断剂氯化镍都能阻断蛇床子素的抑制效应。结论蛇床子素通过抑制钙通道降低蛙的坐骨神经复合动作电位幅度。Objective To investigate the effect of osthole on compound action potential amplitude of frog sciatic nerve fibers and its ionic mechanism.Methods The compound action potential of frog sciatic nerve fibers was studied by extracellular air-gap method.Results The compound action potential amplitude of frog sciatic nerve fibers was decreased in a dose-dependent manner by pretreated osthole.However,the inhibition effect of osthole on amplitude was deceasing in Ca 2+-free medium.Ca 2+channel blockers CdCl 2 or LaCl 3 blocked the inhibition effect of osthole on amplitude.L-type calcium channel blockers diltiazem,verapamil and P/T type calcium channel blockers NiCl can all block the inhibitory effect of osthole.Conclusion Osthole reduces the amplitude of the sciatic nerve complex action potential in frogs by inhibiting calcium channels.

关 键 词：蛙坐骨神经 复合动作电位 蛇床子素 钙通道 

分 类 号：R285[医药卫生—中药学]